Ubiquitin Kit ELISA

N° du produit ABIN1572954

Détail du produit Ubiquitin Kit ELISA

Antigène: Ubiquitin

Reactivité: Cobaye

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of Ub in guinea pig serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of this index.

Réactivité croisée (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 0.36 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Matériel non inclus:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Procédure de l'essai:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Stock: 4 °C,-20 °C

Stockage commentaire: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Date de péremption: 12 months

Détail du antigène

Antigène: Ubiquitin

Autre désignation: Ubiquitin (Ub) (Ubiquitin Produits)

Synonymes: CG11624 Kit ELISA, DmUb Kit ELISA, DmUbi-p63E Kit ELISA, Dmel\\CG11624 Kit ELISA, Ub Kit ELISA, Ubi-P63e Kit ELISA, Ubi-p Kit ELISA, Ubi63E Kit ELISA, Ubi63p Kit ELISA, Ubip Kit ELISA, Ubiq Kit ELISA, Ubp Kit ELISA, ubi Kit ELISA, ubi-p63E Kit ELISA, ubi63E Kit ELISA, CG18282 Kit ELISA, CG32744 Kit ELISA, CR32744 Kit ELISA, DmUbi-p5E Kit ELISA, Dmel\\CG32744 Kit ELISA, ubiquitin Kit ELISA, si:dz180g5.5 Kit ELISA, si:busm1-180g5.5 Kit ELISA, LOC100194618 Kit ELISA, UBIQ Kit ELISA, Ubiquitin Kit ELISA, LOC100282068 Kit ELISA, Ubiquitin-63E Kit ELISA, Ubiquitin-5E Kit ELISA, transmembrane and ubiquitin-like domain containing 2 Kit ELISA, polyubiquitin Kit ELISA, Putative ubiquitin family protein Kit ELISA, ubiquitin Kit ELISA, ubiquitin B Kit ELISA, UBiQuitin Kit ELISA, Ubiquitin Kit ELISA, Ubi-p63E Kit ELISA, Ubi-p5E Kit ELISA, tmub2 Kit ELISA, LOC100194618 Kit ELISA, pco110026 Kit ELISA, LOC100135798 Kit ELISA, UBB Kit ELISA, ubq-1 Kit ELISA, LOC100190766 Kit ELISA

Sujet: Alternative name: Ubiquitin B, UbB, Ubiquitous Immunopoietic Polypeptide, Polyubiquitin B

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway