CNTF Kit ELISA

N° du produit ABIN1672723

Détail du produit CNTF Kit ELISA

Antigène: CNTF (Ciliary Neurotrophic Factor (CNTF))

Épitope: AA 2-200

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CNTF

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: A2-M200

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: A2-M200

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Nervous system.

Plaque: Pre-coated

Protocole: rat CNTF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CNTF has been precoated onto 96-well plates. Standards(E.coli, A2-M200) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CNTF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CNTF amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat CNTF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat CNTF standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 107, Standard deviation: 4.8, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 452, Standard deviation: 18.9, CV(%): 4.2
• Sample 3: n=16, Mean(pg/ml): 1287, Standard deviation: 50.2, CV(%): 3.9,
• Sample 1: n=24, Mean(pg/ml): 193, Standard deviation: 16.2, CV(%): 8.4
• Sample 2: n=24, Mean(pg/ml): 643, Standard deviation: 52.7, CV(%): 8.2
• Sample 3: n=24, Mean(pg/ml): 1057, Standard deviation: 77.2, CV(%): 7.3

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour CNTF Kit ELISA (ABIN1672723)

Sun, Zhu, Yin, Guo, Xu, Xiao, Jiang, Guo, Meng, Lu, Wang, Peng: "Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function." dans: Stem cell research & therapy, Vol. 9, Issue 1, pp. 133, (2018) (PubMed).

Détail du antigène

Antigène: CNTF (Ciliary Neurotrophic Factor (CNTF))

Autre désignation: CNTF (CNTF Produits)

Synonymes: GPA Kit ELISA, CNTF Kit ELISA, HCNTF Kit ELISA, AI429687 Kit ELISA, ciliary neurotrophic factor Kit ELISA, CNTF Kit ELISA, Cntf Kit ELISA

Sujet:

Protein Function: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.

Background: Ciliary neurotrophic factor(CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The hCNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid(aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85 % homology with the deduced aa sequence encoding hCNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.

Synonyms: Ciliary neurotrophic factor,CNTF,Cntf,

Full Gene Name: Ciliary neurotrophic factor

Cellular Localisation: Cytoplasm.

ID gène: 25707

UniProt: P20294

Pathways: Signalistation JAK/STAT