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CXCL6 Kit ELISA

CXCL6 Reactivité: Humain AA 40-114 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1672728
  • Antigène Voir toutes CXCL6 Kits ELISA
    CXCL6 (Chemokine (C-X-C Motif) Ligand 6 (CXCL6))
    Épitope
    AA 40-114
    Reactivité
    • 3
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2-2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL6/GCP2
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: E.coli
    Immunogen sequence: V40-N114
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: E.coli
    Immunogen sequence: V40-N114
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

    Plaque
    Pre-coated
    Protocole
    human CXCL6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL6 has been precoated onto 96-well plates. Standards(E.coli, V40-N114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL6 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CXCL6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL6 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 223, Standard deviation: 13.6, CV(%): 6.1
    • Sample 2: n=16, Mean(pg/ml): 519, Standard deviation: 33.2, CV(%): 6.4
    • Sample 3: n=16, Mean(pg/ml): 1089, Standard deviation: 64.3, CV(%): 5.9,
    • Sample 1: n=24, Mean(pg/ml): 245, Standard deviation: 18.1, CV(%): 7.4
    • Sample 2: n=24, Mean(pg/ml): 542, Standard deviation: 41.2, CV(%): 7.6
    • Sample 3: n=24, Mean(pg/ml): 1054, Standard deviation: 71.7, CV(%): 6.8
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes CXCL6 Kits ELISA
    CXCL6 (Chemokine (C-X-C Motif) Ligand 6 (CXCL6))
    Autre désignation
    CXCL6 (CXCL6 Produits)
    Synonymes
    CKA-3 Kit ELISA, GCP-2 Kit ELISA, GCP2 Kit ELISA, SCYB6 Kit ELISA, Cxcl6 Kit ELISA, CXCL5 Kit ELISA, C-X-C motif chemokine ligand 6 Kit ELISA, chemokine (C-X-C motif) ligand 5 Kit ELISA, CXCL6 Kit ELISA, Cxcl6 Kit ELISA, CXCL5 Kit ELISA
    Sujet

    Protein Function: Chemotactic for neutrophil granulocytes. Signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram- negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7). .

    Background: Chemokine(C-X-C motif) ligand 6(CXCL6) is a small cytokine belonging to the CXC chemokine family that is also known as granulocyte chemotactic protein 2(GCP-2). As its former name suggests, CXCL6 is a chemoattractant for neutrophilic granulocytes. It elicits its chemotactic effects by interacting with the chemokine receptors CXCR1 and CXCR2. The gene for CXCL6 is located on human chromosome 4 in a cluster with other CXC chemokine genes.

    Synonyms: C-X-C motif chemokine 6,Chemokine alpha 3,CKA-3,Granulocyte chemotactic protein 2,GCP-2,Small-inducible cytokine B6,Small-inducible cytokine B6, N-processed variant 1,Small-inducible cytokine B6, N-processed variant 2,Small-inducible cytokine B6, N-processed variant 3,CXCL6,GCP2, SCYB6,

    Full Gene Name: C-X-C motif chemokine 6

    Cellular Localisation: Secreted.
    ID gène
    6372
    UniProt
    P80162
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