TNF alpha Kit ELISA

N° du produit ABIN1672752

Détail du produit TNF alpha Kit ELISA

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Épitope: AA 80-235

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat TNF alpha

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli,L80-L235

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <1pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: L80-L235

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: rat TNF alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNF alpha has been precoated onto 96-well plates. Standards (E.coli,L80-L235) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNF alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TNF alpha amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat TNF alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin , EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat TNF alpha standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 90, Standard deviation: 5.3, CV(%): 5.3
• Sample 2: n=16, Mean(pg/ml): 245, Standard deviation: 3.1, CV(%): 3.1
• Sample 3: n=16, Mean(pg/ml): 612, Standard deviation: 3.4, CV(%): 3.4,
• Sample 1: n=24, Mean(pg/ml): 103, Standard deviation: 6.9, CV(%): 6.7
• Sample 2: n=24, Mean(pg/ml): 261, Standard deviation: 15.4, CV(%): 5.9
• Sample 3: n=24, Mean(pg/ml): 684, Standard deviation: 44.5, CV(%): 6.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour TNF alpha Kit ELISA (ABIN1672752)

Wang, Fu, Yu, Li, Wang, Liu, Zhang, Han, Zhou, Wang: "Preconditioning with VEGF Enhances Angiogenic and Neuroprotective Effects of Bone Marrow Mononuclear Cell Transplantation in a Rat Model of Chronic Cerebral Hypoperfusion." dans: Molecular neurobiology, Vol. 53, Issue 9, pp. 6057-6068, (2018) (PubMed).

Wang, Mao, Yang, Zou, Liu, Liu, Zhang, Xiao, Wang: "Resveratrol protects against early polymicrobial sepsis-induced acute kidney injury through inhibiting endoplasmic reticulum stress-activated NF-κB pathway." dans: Oncotarget, Vol. 8, Issue 22, pp. 36449-36461, (2018) (PubMed).

Piao, Liu, Guo, Meng, Gao: "Picroside II Shows Protective Functions for Severe Acute Pancreatitis in Rats by Preventing NF-κB-Dependent Autophagy." dans: Oxidative medicine and cellular longevity, Vol. 2017, pp. 7085709, (2018) (PubMed).

Chen, Wu, Chen, Xie, Fang, Hu, Chen, Fu, He: "Omega-3 polyunsaturated fatty acid supplementation attenuates microglial-induced inflammation by inhibiting the HMGB1/TLR4/NF-κB pathway following experimental traumatic brain injury." dans: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 143, (2018) (PubMed).

Xiong, Ru, Chen, Tian, Li: "Mitochondrial dysfunction and inflammatory response in the cytotoxicity of NR8383 macrophages induced by fine particulate matter." dans: Environmental toxicology and pharmacology, Vol. 55, pp. 1-7, (2018) (PubMed).

Zhao, He, Pan, Xu, Song: "Kindlin-1 is a key protein in hyperbaric oxygen therapy for the treatment of neuropathic pain." dans: Molecular pain, Vol. 13, pp. 1744806917730250, (2018) (PubMed).

Gao, Meng, Cui: "Budesonide instillation immediately after reperfusion ameliorates ischemia/reperfusion-induced injury in the transplanted lung of rat." dans: Experimental lung research, Vol. 43, Issue 9-10, pp. 439-446, (2018) (PubMed).

Liao, Zheng, Xie, Zhao, Zeng, Liu, Liu: "Adipose tissue-derived stem cells ameliorate hyperglycemia, insulin resistance and liver fibrosis in the type 2 diabetic rats." dans: Stem cell research & therapy, Vol. 8, Issue 1, pp. 286, (2018) (PubMed).

Ling, Feng, Wei, Wang, Wang, Zhang, He, Wang, Zeng, Xiong: "Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats." dans: Stem cell research & therapy, Vol. 8, Issue 1, pp. 283, (2018) (PubMed).

Zhang, Xu, Hu, Wu, Qin, Zhang: "Anti-inflammatory effects of Lefty-1 in renal tubulointerstitial inflammation via regulation of the NF-κB pathway." dans: International journal of molecular medicine, Vol. 41, Issue 3, pp. 1293-1304, (2018) (PubMed).

He, Cui, Wang, Yan, Li, Gong, Zheng, Luo, Wang: "Modulation of inflammation by toll-like receptor 4/nuclear factor-kappa B in diarrhea-predominant irritable bowel syndrome." dans: Oncotarget, Vol. 8, Issue 69, pp. 113957-113965, (2018) (PubMed).

Gao, Jing, Zhang, Wu et al.: "Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide synthase expression and inflammation in rats via the Toll-like receptor ..." dans: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 1029-1035, (2018) (PubMed).

Yamamoto, Yamamoto, Takamura, Usui, Murai, Kaneko, Taniguchi: "Effects of mineralocorticoid receptor antagonists on responses to hemorrhagic shock in rats." dans: World journal of critical care medicine, Vol. 7, Issue 1, pp. 1-8, (2018) (PubMed).

Jing, Luo, Liu, Tian, Zhou: "Fish oil alleviates liver injury induced by intestinal ischemia/reperfusion via AMPK/SIRT-1/autophagy pathway." dans: World journal of gastroenterology, Vol. 24, Issue 7, pp. 833-843, (2018) (PubMed).

Zhu, Guo, Fang, Cao, Yan, Chen, Zhang, Zhang: "Kupffer cell depletion by gadolinium chloride aggravates liver injury after brain death in rats." dans: Molecular medicine reports, Vol. 17, Issue 5, pp. 6357-6362, (2018) (PubMed).

Xu, Qin, Hu, Mu, Zhu, Lu, Luo: "Lentivirus-mediated overexpression of OTULIN ameliorates microglia activation and neuroinflammation by depressing the activation of the NF-κB signaling pathway in cerebral ischemia/reperfusion rats." dans: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 83, (2018) (PubMed).

Sarandy, Miranda, Altoé, Novaes, Zanuncio, Leite, Gonçalves: "Strychnos pseudoquina modulates the morphological reorganization of the scar tissue of second intention cutaneous wounds in rats." dans: PLoS ONE, Vol. 13, Issue 4, pp. e0195786, (2018) (PubMed).

Lin, Ju, Gao, Li, Guo: "Desflurane Attenuates Ventilator-Induced Lung Injury in Rats with Acute Respiratory Distress Syndrome." dans: BioMed research international, Vol. 2018, pp. 7507314, (2018) (PubMed).

Chen, Chen, Fan, Wu, Yang, Fang, Fu, Li et al.: "Omega-3 polyunsaturated fatty acid attenuates the inflammatory response by modulating microglia polarization through SIRT1-mediated deacetylation of the HMGB1/NF-κB pathway following experimental ..." dans: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 116, (2018) (PubMed).

Chen, Ye, Chen, Shi, Wu, Lin, Lin, Li, Fu, Li: "Valproic acid attenuates traumatic spinal cord injury-induced inflammation via STAT1 and NF-κB pathway dependent of HDAC3." dans: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 150, (2018) (PubMed).

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF (TNF alpha Produits)

Synonymes: DIF Kit ELISA, TNF-alpha Kit ELISA, TNFA Kit ELISA, TNFSF2 Kit ELISA, RATTNF Kit ELISA, Tnfa Kit ELISA, tnf Kit ELISA, TNF-a Kit ELISA, TNFalpha Kit ELISA, Tnfsf1a Kit ELISA, TNFa Kit ELISA, cTNF Kit ELISA, Tnf-alpha Kit ELISA, tnfa-like Kit ELISA, TNF-ALPHA Kit ELISA, dif Kit ELISA, tnfa Kit ELISA, xtnf Kit ELISA, tnfsf2 Kit ELISA, tnf-alpha Kit ELISA, Cachectin Kit ELISA, tumor necrosis factor Kit ELISA, tumor necrosis factor b (TNF superfamily, member 2) Kit ELISA, tumor necrosis factor alpha Kit ELISA, tumor necrosis factor a (TNF superfamily, member 2) Kit ELISA, TNF Kit ELISA, Tnf Kit ELISA, tnf Kit ELISA, tnfb Kit ELISA, tnf-alpha Kit ELISA, LOC103694380 Kit ELISA, tnfa Kit ELISA

Sujet:

Protein Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Background: Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30 % amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6. The standard product used in this kit is recombinant rat TNFalpha, consisting of 157 amino acids with the molecular mass of 17KDa.

Synonyms: Tumor necrosis factor,Cachectin,TNF-alpha,Tumor necrosis factor ligand superfamily member 2,TNF-a,Tumor necrosis factor, membrane form,N-terminal fragment,NTF,Intracellular domain 1,ICD1,Intracellular domain 2,ICD2,C-domain 1,C-domain 2,Tumor necrosis factor, soluble form,Tnf,Tnfa, Tnfsf2,

Full Gene Name: Tumor necrosis factor

Cellular Localisation: Cell membrane, Single-pass type II membrane protein.

ID gène: 24835

UniProt: P16599

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome