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Angiopoietin 2 Kit ELISA

ANGPT2 Reactivité: Humain AA 19-496 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum
N° du produit ABIN1672763
  • Antigène Voir toutes Angiopoietin 2 (ANGPT2) Kits ELISA
    Angiopoietin 2 (ANGPT2)
    Épitope
    AA 19-496
    Reactivité
    • 12
    • 9
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156-10000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Angiopoietin-2
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Milk
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: Y19-F496
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: Y19-F496
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Contains 1 fibrinogen C-terminal domain.

    Plaque
    Pre-coated
    Protocole
    human Angiopoietin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Angiopoietin-2 has been precoated onto 96-well plates. Standards(NSO, Y19-F496) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiopoietin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Angiopoietin-2 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Angiopoietin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or human milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Angiopoietin-2 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 1.08, Standard deviation: 0.049, CV(%): 4.5
    • Sample 2: n=16, Mean(ng/ml): 3.49, Standard deviation: 0.181, CV(%): 5.2
    • Sample 3: n=16, Mean(ng/ml): 7.01, Standard deviation: 0.407, CV(%): 5.8,
    • Sample 1: n=24, Mean(ng/ml): 1.05, Standard deviation: 0.071, CV(%): 6.8
    • Sample 2: n=24, Mean(ng/ml): 3.32, Standard deviation: 0.236, CV(%): 7.1
    • Sample 3: n=24, Mean(ng/ml): 6.67, Standard deviation: 0.5, CV(%): 7.5
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • You, Zhuge, Zhu, Si et al.: "Effects of laser photocoagulation on serum angiopoietin-1, angiopoietin-2, angiopoietin-1/angiopoietin-2 ratio, and soluble angiopoietin receptor Tie-2 levels in type 2 diabetic patients with ..." dans: International journal of ophthalmology, Vol. 7, Issue 4, pp. 648-53, (2014) (PubMed).

  • Antigène Voir toutes Angiopoietin 2 (ANGPT2) Kits ELISA
    Angiopoietin 2 (ANGPT2)
    Autre désignation
    ANGPT2 (ANGPT2 Produits)
    Synonymes
    AGPT2 Kit ELISA, ANG2 Kit ELISA, Agpt2 Kit ELISA, Ang-2 Kit ELISA, Ang2 Kit ELISA, ang2 Kit ELISA, angpt2 Kit ELISA, ANGIOPOIETIN-2 Kit ELISA, ANG-2 Kit ELISA, ANGPT2 Kit ELISA, Angrp Kit ELISA, Rnase5b Kit ELISA, ANG3 Kit ELISA, C11orf2 Kit ELISA, C11orf3 Kit ELISA, FFR Kit ELISA, angiopoietin 2 Kit ELISA, angiopoietin 2b Kit ELISA, angiogenin, ribonuclease A family, member 2 Kit ELISA, VPS51, GARP complex subunit Kit ELISA, ANGPT2 Kit ELISA, Angpt2 Kit ELISA, angpt2b Kit ELISA, Ang2 Kit ELISA, VPS51 Kit ELISA
    Sujet

    Protein Function: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. .

    Background: Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

    Synonyms: Angiopoietin-2,ANG-2,ANGPT2,

    Full Gene Name: Angiopoietin-2

    Cellular Localisation: Secreted.
    ID gène
    285
    UniProt
    O15123
    Pathways
    Signalisation RTK
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