Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

CCL18 Kit ELISA

CCL18 Reactivité: Humain AA 21-89 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Serum
N° du produit ABIN1672771
  • Antigène Voir toutes CCL18 Kits ELISA
    CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))
    Épitope
    AA 21-89
    Reactivité
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    7.8-500 pg/mL
    Seuil minimal de détection
    7.8 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL18/PARC
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: E.coli
    Immunogen sequence: A21-A89
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: E.coli
    Immunogen sequence: A21-A89
    Featured
    Discover our best selling CCL18 Kit ELISA
    Top Product
    Discover our top product CCL18 Kit ELISA
  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

    Tissue Specificity: Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid. .

    Plaque
    Pre-coated
    Protocole
    human CCL18 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL18 has been precoated onto 96-well plates. Standards(E.coli, A21-A89) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL18 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human CCL18 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human CCL18 standard solution and each sample is measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 49, Standard deviation: 1.62, CV(%): 3.3
    • Sample 2: n=16, Mean(pg/ml): 199, Standard deviation: 7.36, CV(%): 3.7
    • Sample 3: n=16, Mean(pg/ml): 346, Standard deviation: 14.88, CV(%): 4.3,
    • Sample 1: n=24, Mean(pg/ml): 53, Standard deviation: 3.66, CV(%): 6.9
    • Sample 2: n=24, Mean(pg/ml): 218, Standard deviation: 15.70, CV(%): 7.2
    • Sample 3: n=24, Mean(pg/ml): 362, Standard deviation: 27.87, CV(%): 7.7
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes CCL18 Kits ELISA
    CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))
    Autre désignation
    CCL18 (CCL18 Produits)
    Synonymes
    H7AP1 Kit ELISA, PARC Kit ELISA, RP3-330M21.2 Kit ELISA, AMAC-1 Kit ELISA, AMAC1 Kit ELISA, CKb7 Kit ELISA, DC-CK1 Kit ELISA, DCCK1 Kit ELISA, MIP-4 Kit ELISA, SCYA18 Kit ELISA, cullin 9 Kit ELISA, C-C motif chemokine ligand 18 Kit ELISA, CUL9 Kit ELISA, CCL18 Kit ELISA
    Sujet

    Protein Function: Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses. .

    Background: Chemokine(C-C motif) ligand 18(CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC(pulmonary and activation-regulated chemokine). CCL18 is approximately 60 % identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma.

    Synonyms: C-C motif chemokine 18,Alternative macrophage activation-associated CC chemokine 1,AMAC-1,CC chemokine PARC,Dendritic cell chemokine 1,DC-CK1,Macrophage inflammatory protein 4,MIP-4,Pulmonary and activation-regulated chemokine,Small-inducible cytokine A18,CCL18(1-68),CCL18(3-69),CCL18(4-69),CCL18,AMAC1, DCCK1, MIP4, PARC, SCYA18,

    Full Gene Name: C-C motif chemokine 18

    Cellular Localisation: Secreted.
    ID gène
    6362
    UniProt
    P55774
Vous êtes ici:
Support technique