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CD27 Kit ELISA

CD27 Reactivité: Souris AA 21-182 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
N° du produit ABIN1672775
  • Antigène Voir toutes CD27 Kits ELISA
    CD27 (CD27 Molecule (CD27))
    Épitope
    AA 21-182
    Reactivité
    • 6
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    62.5-4000 pg/mL
    Seuil minimal de détection
    62.5 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFRSF7/CD27
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: T21-R182
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: T21-R182
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    Discover our top product CD27 Kit ELISA
  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: In thymus and spleen, but not in non-lymphoid tissues.

    Plaque
    Pre-coated
    Protocole
    mouse TNFRSF7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF7 has been precoated onto 96-well plates. Standards(NSO, T21-R182) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF7 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse TNFRSF7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates and serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFRSF7 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 533, Standard deviation: 22.4, CV(%): 4.2
    • Sample 2: n=16, Mean(pg/ml): 1626, Standard deviation: 56.91, CV(%): 3.5
    • Sample 3: n=16, Mean(pg/ml): 2563, Standard deviation: 138.4, CV(%): 5.4,
    • Sample 1: n=24, Mean(pg/ml): 658, Standard deviation: 39.5, CV(%): 6
    • Sample 2: n=24, Mean(pg/ml): 1856, Standard deviation: 89.1, CV(%): 4.8
    • Sample 3: n=24, Mean(pg/ml): 2731, Standard deviation: 180.3, CV(%): 6.6
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes CD27 Kits ELISA
    CD27 (CD27 Molecule (CD27))
    Autre désignation
    CD27 (CD27 Produits)
    Synonymes
    TNFRSF7 Kit ELISA, S152 Kit ELISA, Tnfrsf7 Kit ELISA, Tp55 Kit ELISA, S152. LPFS2 Kit ELISA, T14 Kit ELISA, CD27 molecule Kit ELISA, CD27 antigen Kit ELISA, CD27 Kit ELISA, Cd27 Kit ELISA
    Sujet

    Protein Function: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

    Background: CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein(SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

    Synonyms: CD27 antigen,CD27L receptor,T-cell activation antigen CD27,Tumor necrosis factor receptor superfamily member 7,CD27,Cd27,Tnfrsf7,

    Full Gene Name: CD27 antigen

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    ID gène
    21940
    UniProt
    P41272
    Pathways
    Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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