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CD80 Kit ELISA

CD80 Reactivité: Humain AA 35-242 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
N° du produit ABIN1672777
  • Antigène Voir toutes CD80 Kits ELISA
    CD80
    Épitope
    AA 35-242
    Reactivité
    • 6
    • 6
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    62.5-4000 pg/mL
    Seuil minimal de détection
    62.5 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human B7-1/CD80
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: V35-N242
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: V35-N242
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

    Tissue Specificity: Expressed on activated B-cells, macrophages and dendritic cells.

    Plaque
    Pre-coated
    Protocole
    human CD80 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD80 has been precoated onto 96-well plates. Standards(NSO, V35-N242) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD80 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CD80 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD80 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 0.22, Standard deviation: 0.013, CV(%): 5.7
    • Sample 2: n=16, Mean(ng/ml): 1.47, Standard deviation: 0.090, CV(%): 6.1
    • Sample 3: n=16, Mean(ng/ml): 2.34, Standard deviation: 0.185, CV(%): 7.9,
    • Sample 1: n=24, Mean(ng/ml): 0.27, Standard deviation: 0.018, CV(%): 6.8
    • Sample 2: n=24, Mean(ng/ml): 1.56, Standard deviation: 0.115, CV(%): 7.4
    • Sample 3: n=24, Mean(ng/ml): 2.58, Standard deviation: 0.214, CV(%): 8.3
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes CD80 Kits ELISA
    CD80
    Autre désignation
    CD80 (CD80 Produits)
    Synonymes
    B7 Kit ELISA, B7-1 Kit ELISA, B7.1 Kit ELISA, BB1 Kit ELISA, CD28LG Kit ELISA, CD28LG1 Kit ELISA, LAB7 Kit ELISA, B71 Kit ELISA, Cd28l Kit ELISA, Ly-53 Kit ELISA, Ly53 Kit ELISA, MIC17 Kit ELISA, TSA1 Kit ELISA, CD80 molecule Kit ELISA, Cd80 molecule Kit ELISA, CD80 antigen Kit ELISA, CD80 Kit ELISA, Cd80 Kit ELISA
    Sujet

    Protein Function: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. .

    Background: The protein CD80(Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

    Synonyms: T-lymphocyte activation antigen CD80,Activation B7-1 antigen,BB1,CTLA-4 counter-receptor B7.1,B7,CD80,CD80,CD28LG, CD28LG1, LAB7,

    Full Gene Name: T-lymphocyte activation antigen CD80

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    ID gène
    941
    UniProt
    P33681
    Pathways
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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