PF4 Kit ELISA

N° du produit ABIN1672782

Détail du produit PF4 Kit ELISA

Antigène: PF4 (Platelet Factor 4 (PF4))

Épitope: AA 30-105

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CXCL4/PF4

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: V30-S105

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <1pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: V30-S105

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse CXCL4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL4 has been precoated onto 96-well plates. Standards(E.coli, V30-S105) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL4 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CXCL4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CXCL4 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 84.2, Standard deviation: 4.97, CV(%): 5.9
• Sample 2: n=16, Mean(pg/ml): 266, Standard deviation: 16.49, CV(%): 6.2
• Sample 3: n=16, Mean(pg/ml): 524, Standard deviation: 25.2, CV(%): 4.8,
• Sample 1: n=24, Mean(pg/ml): 92.1, Standard deviation: 6.63, CV(%): 7.2
• Sample 2: n=24, Mean(pg/ml): 285, Standard deviation: 21.09, CV(%): 7.4
• Sample 3: n=24, Mean(pg/ml): 519, Standard deviation: 31.7, CV(%): 6.1

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: PF4 (Platelet Factor 4 (PF4))

Autre désignation: CXCL4 (PF4 Produits)

Synonymes: CXCL4 Kit ELISA, PF-4 Kit ELISA, SCYB4 Kit ELISA, Cxcl4 Kit ELISA, Scyb4 Kit ELISA, Pf4a Kit ELISA, RATPF4A Kit ELISA, PF4A Kit ELISA, CXCL4L1 Kit ELISA, CXCL4V1 Kit ELISA, PF4-ALT Kit ELISA, SCYB4V1 Kit ELISA, PF4 Kit ELISA, platelet factor 4 Kit ELISA, platelet factor 4 variant 1 Kit ELISA, PF4 Kit ELISA, Pf4 Kit ELISA, LOC703451 Kit ELISA, PF4V1 Kit ELISA, LOC100524373 Kit ELISA, LOC102181854 Kit ELISA, LOC100630489 Kit ELISA

Sujet:

Protein Function: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation (By similarity). .

Background: Platelet factor 4(PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine(C-X-C motif) ligand 4(CXCL4). The PF4 gene was localized on map to 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

Synonyms: Platelet factor 4,PF-4,C-X-C motif chemokine 4,Pf4,Cxcl4, Scyb4,

Full Gene Name: Platelet factor 4

Cellular Localisation: Secreted.

ID gène: 56744

UniProt: Q9Z126