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GDF15 Kit ELISA

GDF15 Reactivité: Humain AA 197-308 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1672794
  • Antigène Voir toutes GDF15 Kits ELISA
    GDF15 (Growth Differentiation Factor 15 (GDF15))
    Épitope
    AA 197-308
    Reactivité
    • 11
    • 7
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2-2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human GDF-15
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: CHO
    Immunogen sequence: A197-I308
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: CHO
    Immunogen sequence: A197-I308
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the TGF-beta family.

    Tissue Specificity: Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.

    Plaque
    Pre-coated
    Protocole
    human GDF-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GDF-15 has been precoated onto 96-well plates. Standards(CHO, A197-I308) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDF-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GDF-15 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human GDF-15 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human GDF-15 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 104, Standard deviation: 2.5, CV(%): 2.4
    • Sample 2: n=16, Mean(pg/ml): 735, Standard deviation: 27.93, CV(%): 3.8
    • Sample 3: n=16, Mean(pg/ml): 1518, Standard deviation: 68.31, CV(%): 4.5,
    • Sample 1: n=24, Mean(pg/ml): 121, Standard deviation: 6.897, CV(%): 5.7
    • Sample 2: n=24, Mean(pg/ml): 757, Standard deviation: 46.18, CV(%): 6.1
    • Sample 3: n=24, Mean(pg/ml): 1526, Standard deviation: 112.9, CV(%): 7.4
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes GDF15 Kits ELISA
    GDF15 (Growth Differentiation Factor 15 (GDF15))
    Autre désignation
    GDF15 (GDF15 Produits)
    Synonymes
    GDF15 Kit ELISA, GDF-15 Kit ELISA, MIC-1 Kit ELISA, MIC1 Kit ELISA, NAG-1 Kit ELISA, PDF Kit ELISA, PLAB Kit ELISA, PTGFB Kit ELISA, SBF Kit ELISA, growth differentiation factor 15 Kit ELISA, GDF15 Kit ELISA, Gdf15 Kit ELISA
    Sujet

    Background: GDF-15(Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.

    Synonyms: Growth/differentiation factor 15,GDF-15,Macrophage inhibitory cytokine 1,MIC-1,NSAID-activated gene 1 protein,NAG-1,NSAID-regulated gene 1 protein,NRG-1,Placental TGF-beta,Placental bone morphogenetic protein,Prostate differentiation factor,GDF15,MIC1, PDF, PLAB, PTGFB,

    Full Gene Name: Growth/differentiation factor 15

    Cellular Localisation: Secreted.
    ID gène
    9518
    UniProt
    Q99988
    Pathways
    SARS-CoV-2 Protein Interactome
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