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Kallikrein 5 Kit ELISA

KLK5 Reactivité: Humain AA 23-293 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum
N° du produit ABIN1672807
  • Antigène Voir toutes Kallikrein 5 (KLK5) Kits ELISA
    Kallikrein 5 (KLK5)
    Épitope
    AA 23-293
    Reactivité
    • 9
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    78-5000 pg/mL
    Seuil minimal de détection
    78 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human KLK5
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Milk, Saliva
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: V23-S293
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: V23-S293
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the peptidase S1 family. Kallikrein subfamily.

    Tissue Specificity: Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin. .

    Plaque
    Pre-coated
    Protocole
    human KLK5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK5 has been precoated onto 96-well plates. Standards(NSO, V23-S293) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK5 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human KLK5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), human milk or saliva to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human KLK5 standard solution and each sample is measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 545, Standard deviation: 38.7, CV(%): 7.1
    • Sample 2: n=16, Mean(pg/ml): 1823, Standard deviation: 69.3, CV(%): 3.8
    • Sample 3: n=16, Mean(pg/ml): 3240, Standard deviation: 158.8, CV(%): 4.9,
    • Sample 1: n=24, Mean(pg/ml): 613, Standard deviation: 46.6, CV(%): 7.6
    • Sample 2: n=24, Mean(pg/ml): 1972, Standard deviation: 98.6, CV(%): 5
    • Sample 3: n=24, Mean(pg/ml): 3468, Standard deviation: 260.1, CV(%): 7.5
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes Kallikrein 5 (KLK5) Kits ELISA
    Kallikrein 5 (KLK5)
    Autre désignation
    KLK5 (KLK5 Produits)
    Synonymes
    KLK-L2 Kit ELISA, KLKL2 Kit ELISA, SCTE Kit ELISA, 1110030O19Rik Kit ELISA, KAL Kit ELISA, KALA Kit ELISA, Klk1 Kit ELISA, Klk5 Kit ELISA, RATKALA Kit ELISA, kallikrein related peptidase 5 Kit ELISA, kallikrein related-peptidase 5 Kit ELISA, kallikrein 5-like Kit ELISA, KLK5 Kit ELISA, Klk5 Kit ELISA, Klk5l Kit ELISA
    Sujet

    Protein Function: May be involved in desquamation.

    Background: Kallikrein gene 5(KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding(desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.

    Synonyms: Kallikrein-5,3.4.21.-,Kallikrein-like protein 2,KLK-L2,Stratum corneum tryptic enzyme,KLK5,SCTE,UNQ570/PRO1132,

    Full Gene Name: Kallikrein-5

    Cellular Localisation: Secreted.
    ID gène
    25818
    UniProt
    Q9Y337
    Pathways
    Système du Complément, Regulation of G-Protein Coupled Receptor Protein Signaling
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