BMP7 Kit ELISA

N° du produit ABIN1672823

Détail du produit BMP7 Kit ELISA

Antigène: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Épitope: AA 293-431

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human BMP-7

Marque: PicoKine™

Type d'échantillon: Osseous Tissue, Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: S293-H431

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: S293-H431

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: human BMP-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-7 has been precoated onto 96-well plates. Standards(NSO, S293-H431) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BMP-7 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human BMP-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human bone tissue, cell culture supernates, serum, plasma((heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human BMP-7 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 342, Standard deviation: 19.8, CV(%): 5.8
• Sample 2: n=16, Mean(pg/ml): 765, Standard deviation: 42.8, CV(%): 5.6
• Sample 3: n=16, Mean(pg/ml): 1108, Standard deviation: 57.6, CV(%): 5.2,
• Sample 1: n=24, Mean(pg/ml): 372, Standard deviation: 30.5, CV(%): 8.2
• Sample 2: n=24, Mean(pg/ml): 780, Standard deviation: 62.4, CV(%): 8
• Sample 3: n=24, Mean(pg/ml): 1265, Standard deviation: 98.7, CV(%): 7.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour BMP7 Kit ELISA (ABIN1672823)

Wang, Xue, Zhao, Liu, Ma, Ma: "Form-deprivation myopia induces decreased expression of bone morphogenetic protein-2, 5 in guinea pig sclera." dans: International journal of ophthalmology, Vol. 8, Issue 1, pp. 39-45, (2015) (PubMed).

Li, Yi, Jin, Duan, Min: "Effects of sequentially released BMP-2 and BMP-7 from PELA microcapsule-based scaffolds on the bone regeneration." dans: American journal of translational research, Vol. 7, Issue 8, pp. 1417-28, (2015) (PubMed).

Qu, Du, Zhang, Li, Han, Qiu: "Changes in the expression of bone morphogenetic protein 7 and tamm- horsfall protein in the early stages of diabetic nephropathy." dans: Nephro-urology monthly, Vol. 4, Issue 2, pp. 466-9, (2013) (PubMed).

Tu, Dong, Lu: "Effects of Panax notoginoside on the nephropathy in rats with type 1 diabetes mellitus." dans: Chinese journal of integrative medicine, Vol. 17, Issue 8, pp. 612-5, (2011) (PubMed).

Che, Zhang, Li, Tan, Bai, Qu: "Application of tissue-engineered cartilage with BMP-7 gene to repair knee joint cartilage injury in rabbits." dans: Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA, Vol. 18, Issue 4, pp. 496-503, (2010) (PubMed).

Détail du antigène

Antigène: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Autre désignation: BMP7 (BMP7 Produits)

Synonymes: bmp-7 Kit ELISA, bmp7 Kit ELISA, op-1 Kit ELISA, BMP7 Kit ELISA, zgc:153025 Kit ELISA, OP-1 Kit ELISA, OP1 Kit ELISA, BMP-7 Kit ELISA, fb83f09 Kit ELISA, wu:fb83f09 Kit ELISA, bone morphogenetic protein 7 Kit ELISA, bone morphogenetic protein 7, gene 1 L homeolog Kit ELISA, bone morphogenetic protein 7b Kit ELISA, bone morphogenetic protein 7, gene 2 L homeolog Kit ELISA, bone morphogenetic protein 7a Kit ELISA, BMP7 Kit ELISA, bmp7.1.L Kit ELISA, LOC100136282 Kit ELISA, bmp7b Kit ELISA, Bmp7 Kit ELISA, LOC100008826 Kit ELISA, bmp7.2.L Kit ELISA, bmp7a Kit ELISA

Sujet:

Background: Bone morphogenetic protein 7 or BMP7(also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Synonyms: Bone morphogenetic protein 7 (Osteogenic protein 1), isoform CRA_b ,cDNA FLJ78019, highly similar to Homo sapiens bone morphogenetic protein 7 (osteogenic protein 1), mRNA ,cDNA, FLJ92758, Homo sapiens bone morphogenetic protein 7 (osteogenic protein 1)(BMP7), mRNA ,BMP7 ,hCG_40100 ,

Full Gene Name: bone morphogenetic protein 7

ID gène: 655

UniProt: A8K571

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance