Clusterin Kit ELISA

N° du produit ABIN1672832

Détail du produit Clusterin Kit ELISA

Antigène: Clusterin (CLU)

Épitope: AA 23-227, AA 228-449

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78-50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Clusterin

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: D23-R227(beta)&S228-E449(alpha)

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <20pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: D23-R227(beta)&S228-E449(alpha)

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the clusterin family.

Tissue Specificity: Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung. .

Plaque: Pre-coated

Protocole: human Clusterin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Clusterin has been precoated onto 96-well plates. Standards(NSO, D23-R227(β)&S228-E449(α)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Clusterin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Clusterin amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Clusterin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Clusterin standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 5.62, Standard deviation: 0.259, CV(%): 4.6
• Sample 2: n=16, Mean(ng/ml): 10.1, Standard deviation: 0.444, CV(%): 4.4
• Sample 3: n=16, Mean(ng/ml): 25.5, Standard deviation: 1.07, CV(%): 4.2,
• Sample 1: n=24, Mean(ng/ml): 6.23, Standard deviation: 0.467, CV(%): 7.5
• Sample 2: n=24, Mean(ng/ml): 12.1, Standard deviation: 0.835, CV(%): 6.9
• Sample 3: n=24, Mean(ng/ml): 26.4, Standard deviation: 1.95, CV(%): 7.4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour Clusterin Kit ELISA (ABIN1672832)

Kim, Song, Kim, Jeon, Kim, Kang, Chun, Hong, Chung, Kim, Kim, Kim: "Urine clusterin/apolipoprotein J is linked to tubular damage and renal outcomes in patients with type 2 diabetes mellitus." dans: Clinical endocrinology, Vol. 87, Issue 2, pp. 156-164, (2018) (PubMed).

Song, Zhang, Zhang, Du, Guo, Kuang, Wang, Wu, Zou, Zou, Lv, Wang: "A microfluidic device for studying chemotaxis mechanism of bacterial cancer targeting." dans: Scientific reports, Vol. 8, Issue 1, pp. 6394, (2018) (PubMed).

Zheng, Yao, Qian, Sai, Qiu, Yang, Wu, Dong, Yao: "Oncogenic secretory clusterin in hepatocellular carcinoma: Expression at early staging and emerging molecular target." dans: Oncotarget, Vol. 8, Issue 32, pp. 52321-52332, (2017) (PubMed).

Détail du antigène

Antigène: Clusterin (CLU)

Autre désignation: CLU (CLU Produits)

Synonymes: LOC100136120 Kit ELISA, clu Kit ELISA, MGC53055 Kit ELISA, zgc:73352 Kit ELISA, xclu Kit ELISA, MGC89163 Kit ELISA, CLU Kit ELISA, APO-J Kit ELISA, APOJ Kit ELISA, CLI Kit ELISA, CLU1 Kit ELISA, CLU2 Kit ELISA, KUB1 Kit ELISA, NA1/NA2 Kit ELISA, SGP-2 Kit ELISA, SGP2 Kit ELISA, SP-40 Kit ELISA, TRPM-2 Kit ELISA, TRPM2 Kit ELISA, DAG Kit ELISA, RATTRPM2B Kit ELISA, SP40 Kit ELISA, TRPM2B Kit ELISA, Trpm2 Kit ELISA, Trpmb Kit ELISA, AI893575 Kit ELISA, ApoJ Kit ELISA, Cli Kit ELISA, D14Ucla3 Kit ELISA, Sgp-2 Kit ELISA, Sgp2 Kit ELISA, Sugp-2 Kit ELISA, GP80 Kit ELISA, gpIII Kit ELISA, 40 Kit ELISA, clusterin-1 Kit ELISA, clusterin L homeolog Kit ELISA, clusterin Kit ELISA, Clusterin Kit ELISA, LOC100136120 Kit ELISA, clu.L Kit ELISA, clu Kit ELISA, CLU Kit ELISA, clus Kit ELISA, Clu Kit ELISA, LOC101113728 Kit ELISA

Sujet:

Protein Function: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress- induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. .

Background: Clusterin(apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis.[1] In humans, clusterin is encoded by the CLU gene.This protein has several Synonyms: dimeric acidic glycoprotein(DAG protein), testosterone repressed prostate message-2(TRPM-2), sulfated glycoprotein-2(SGP-2) and complement lysis inhibitor(CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic(H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.

Synonyms: Clusterin,Aging-associated gene 4 protein,Apolipoprotein J,Apo-J,Complement cytolysis inhibitor,CLI,Complement-associated protein SP-40,40,Ku70-binding protein 1,NA1/NA2,Testosterone-repressed prostate message 2,TRPM-2,Clusterin beta chain,ApoJalpha,Complement cytolysis inhibitor a chain,Clusterin alpha chain,ApoJbeta,Complement cytolysis inhibitor b chain,CLU,APOJ, CLI, KUB1,AAG4,

Full Gene Name: Clusterin

Cellular Localisation: Isoform 1: Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress.

ID gène: 1191

UniProt: P10909

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling