EGF Kit ELISA

N° du produit ABIN1672859

Détail du produit EGF Kit ELISA

Antigène: EGF (Epidermal Growth Factor (EGF))

Épitope: AA 974-1026

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.8-500 pg/mL

Seuil minimal de détection: 7.8 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat EGF

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate, Urine

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: N974-R1026

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <1pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: N974-R1026

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 9 EGF-like domains.

Plaque: Pre-coated

Protocole: rat EGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EGF has been precoated onto 96-well plates. Standards(E.coli, N974-R1026) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat EGF amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL rat EGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat EGF standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 32.1, Standard deviation: 1.35, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 104, Standard deviation: 5.62, CV(%): 5.4
• Sample 3: n=16, Mean(pg/ml): 256, Standard deviation: 11.52, CV(%): 4.5,
• Sample 1: n=24, Mean(pg/ml): 35.2, Standard deviation: 2.36, CV(%): 6.7
• Sample 2: n=24, Mean(pg/ml): 112, Standard deviation: 8.4, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 287, Standard deviation: 18.08, CV(%): 6.3

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour EGF Kit ELISA (ABIN1672859)

Li, Wu, Chen, Chen, Xu, Gu, Song: "Antiplatelet drug ticagrelor delays gastric ulcer healing in rats." dans: Experimental and therapeutic medicine, Vol. 14, Issue 4, pp. 3774-3779, (2017) (PubMed).

Yang, Wu, Feng, Huang, Liu, Liu, Chen: "Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats." dans: Stem cell research & therapy, Vol. 8, Issue 1, pp. 267, (2017) (PubMed).

Russo, Linsalata, Clemente, DAttoma, Orlando, Campanella, Giotta, Riezzo: "The effects of fluorouracil, epirubicin, and cyclophosphamide (FEC60) on the intestinal barrier function and gut peptides in breast cancer patients: an observational study." dans: BMC cancer, Vol. 13, pp. 56, (2013) (PubMed).

Ge, Yu, Petitte, Zhang: "Epidermal growth factor-induced proliferation of chicken primordial germ cells: involvement of calcium/protein kinase C and NFKB1." dans: Biology of reproduction, Vol. 80, Issue 3, pp. 528-36, (2009) (PubMed).

Détail du antigène

Antigène: EGF (Epidermal Growth Factor (EGF))

Autre désignation: EGF (EGF Produits)

Synonymes: HOMG4 Kit ELISA, URG Kit ELISA, AI790464 Kit ELISA, CEGF Kit ELISA, epidermal growth factor Kit ELISA, pro-epidermal growth factor Kit ELISA, EGF Kit ELISA, egf Kit ELISA, CpipJ_CPIJ020278 Kit ELISA, Egf Kit ELISA

Sujet:

Protein Function: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity). .

Background: Epidermal growth factor(EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays a physiological role in the maintenance of oro-esophageal and gastric tissue integrity.

Synonyms: Pro-epidermal growth factor,EGF,Epidermal growth factor,Egf,

Full Gene Name: Pro-epidermal growth factor

Cellular Localisation: Membrane, Single-pass type I membrane protein.

ID gène: 25313

UniProt: P07522

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation