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Thymic Stromal Lymphopoietin Kit ELISA

TSLP Reactivité: Humain AA 29-159 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1672862
  • Antigène Voir toutes Thymic Stromal Lymphopoietin (TSLP) Kits ELISA
    Thymic Stromal Lymphopoietin (TSLP)
    Épitope
    AA 29-159
    Reactivité
    • 12
    • 8
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2-2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TSLP
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: E.coli
    Immunogen sequence: Y29-Q159
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: E.coli
    Immunogen sequence: Y29-Q159
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: Isoform 1 is expressed in a number of tissues including heart, liver and prostate. Isoform 2 is the predominant form in keratinocytes of oral mucosa, skin and in salivary glands. It is secreted into saliva. .

    Plaque
    Pre-coated
    Protocole
    human TSLP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TSLP has been precoated onto 96-well plates. Standards(E.coli, Y29-Q159) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TSLP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TSLP amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human TSLP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TSLP standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 258, Standard deviation: 23.99, CV(%): 9.3
    • Sample 2: n=16, Mean(pg/ml): 762, Standard deviation: 57.15, CV(%): 7.5
    • Sample 3: n=16, Mean(pg/ml): 1229, Standard deviation: 103.24, CV(%): 8.4,
    • Sample 1: n=24, Mean(pg/ml): 262, Standard deviation: 25.41, CV(%): 9.7
    • Sample 2: n=24, Mean(pg/ml): 770, Standard deviation: 58.52, CV(%): 7.6
    • Sample 3: n=24, Mean(pg/ml): 1335, Standard deviation: 117.48, CV(%): 8.8
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes Thymic Stromal Lymphopoietin (TSLP) Kits ELISA
    Thymic Stromal Lymphopoietin (TSLP)
    Autre désignation
    TSLP (TSLP Produits)
    Synonymes
    TSLP Kit ELISA, thymic stromal lymphopoietin Kit ELISA, TSLP Kit ELISA, Tslp Kit ELISA
    Sujet

    Protein Function: Isoform 1: Cytokine that induces the release of T-cell- attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells. .

    Background: Thymic stromal lymphopoietin, also called TSLP is a protein belonging to the cytokine family. This gene is mapped to 5q22.1. It encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2(TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases.

    Synonyms: Thymic stromal lymphopoietin,TSLP,

    Full Gene Name: Thymic stromal lymphopoietin

    Cellular Localisation: Secreted.
    ID gène
    85480
    UniProt
    Q969D9
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