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Betacellulin Kit ELISA

BTC Reactivité: Humain AA 32-111 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1672881
  • Antigène Voir toutes Betacellulin (BTC) Kits ELISA
    Betacellulin (BTC)
    Épitope
    AA 32-111
    Reactivité
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.6-1000 pg/mL
    Seuil minimal de détection
    15.6 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Betacellulin/BTC
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: E.coli
    Immunogen sequence: D32-Y111
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: E.coli
    Immunogen sequence: D32-Y111
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: Synthesized in several tissues and tumor cells. Predominantly expressed in pancreas and small intestine. .

    Plaque
    Pre-coated
    Protocole
    human Betacellulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Betacellulin has been precoated onto 96-well plates. Standards(E.coli, D32-Y111) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Betacellulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Betacellulin amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human Betacellulin standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Betacellulin standard solution and each sample is measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 46, Standard deviation: 2.35, CV(%): 5.1
    • Sample 2: n=16, Mean(pg/ml): 119, Standard deviation: 6.31, CV(%): 5.3
    • Sample 3: n=16, Mean(pg/ml): 725, Standard deviation: 45.68, CV(%): 6.3,
    • Sample 1: n=24, Mean(pg/ml): 47, Standard deviation: 2.73, CV(%): 5.8
    • Sample 2: n=24, Mean(pg/ml): 124, Standard deviation: 7.69, CV(%): 6.2
    • Sample 3: n=24, Mean(pg/ml): 736, Standard deviation: 58.14, CV(%): 7.9
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes Betacellulin (BTC) Kits ELISA
    Betacellulin (BTC)
    Autre désignation
    BTC (BTC Produits)
    Synonymes
    BTC Kit ELISA, LOC100228121 Kit ELISA, probetacellulin Kit ELISA, Bcn Kit ELISA, betacellulin Kit ELISA, betacellulin, epidermal growth factor family member Kit ELISA, BTC Kit ELISA, LOC100228121 Kit ELISA, Btc Kit ELISA
    Sujet

    Protein Function: Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. .

    Background: Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

    Synonyms: Probetacellulin,Betacellulin,BTC,BTC,

    Full Gene Name: Probetacellulin

    Cellular Localisation: Betacellulin: Secreted, extracellular space.
    ID gène
    685
    UniProt
    P35070
    Pathways
    Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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