ICAM2 Kit ELISA

N° du produit ABIN1672894

Détail du produit ICAM2 Kit ELISA

Antigène: ICAM2 (Intercellular Adhesion Molecule 2 (ICAM2))

Épitope: AA 25-223

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312-20000 pg/mL

Seuil minimal de détection: 312 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ICAM-2

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: K25-Q223

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: K25-Q223

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the immunoglobulin superfamily. ICAM family.

Plaque: Pre-coated

Protocole: human ICAM-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ICAM-2 has been precoated onto 96-well plates. Standards(NSO, K25-Q223) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ICAM-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ICAM-2 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human ICAM-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, tissue homogenates, serum or plasma( heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ICAM-2 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 1.56, Standard deviation: 0.066, CV(%): 4.2
• Sample 2: n=16, Mean(ng/ml): 7.62, Standard deviation: 0.343, CV(%): 4.5
• Sample 3: n=16, Mean(ng/ml): 13.3, Standard deviation: 0.705, CV(%): 5.3,
• Sample 1: n=24, Mean(ng/ml): 1.36, Standard deviation: 0.078, CV(%): 5.7
• Sample 2: n=24, Mean(ng/ml): 7.53, Standard deviation: 0.152, CV(%): 6.8
• Sample 3: n=24, Mean(ng/ml): 14.8, Standard deviation: 1.11, CV(%): 7.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour ICAM2 Kit ELISA (ABIN1672894)

Xu, Feng, Wang, Zhu, Lin, Lou, Xiang, He, Zheng, Tang, Zuo: "Phytoestrogen calycosin-7-O-?-D-glucopyranoside ameliorates advanced glycation end products-induced HUVEC damage." dans: Journal of cellular biochemistry, Vol. 112, Issue 10, pp. 2953-65, (2011) (PubMed).

Détail du antigène

Antigène: ICAM2 (Intercellular Adhesion Molecule 2 (ICAM2))

Autre désignation: ICAM2 (ICAM2 Produits)

Synonymes: ICAM2 Kit ELISA, CD102 Kit ELISA, Icam-2 Kit ELISA, Ly-60 Kit ELISA, intercellular adhesion molecule 2 Kit ELISA, LOC506088 Kit ELISA, ICAM2 Kit ELISA, LOC616254 Kit ELISA, LOC100140873 Kit ELISA, Icam2 Kit ELISA

Sujet:

Protein Function: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen- specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.

Background: Intercellular adhesion molecule 2(ICAM2), also known as CD102(Cluster of Differentiation 102) is an integral membrane protein that has 2 immunoglobulin-like domains. This gene is mapped to 17q23.3. The protein encoded by this gene is a member of the intercellular adhesion molecule(ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.

Synonyms: Intercellular adhesion molecule 2,ICAM-2,CD102,ICAM2,

Full Gene Name: Intercellular adhesion molecule 2

Cellular Localisation: Membrane, Single-pass type I membrane protein.

ID gène: 3384

UniProt: P13598