PI3 Kit ELISA

N° du produit ABIN1672903

Détail du produit PI3 Kit ELISA

Antigène: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Épitope: AA 23-117

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Elafin/PI3

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: A23-Q117

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: A23-Q117

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: human Elafin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Elafin has been precoated onto 96-well plates. Standards(NSO, A23-Q117) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Elafin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Elafin amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Elafin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Elafin standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 225, Standard deviation: 12.6, CV(%): 5.6
• Sample 2: n=16, Mean(pg/ml): 657, Standard deviation: 32.2, CV(%): 4.9
• Sample 3: n=16, Mean(pg/ml): 1334, Standard deviation: 57.36, CV(%): 4.3,
• Sample 1: n=24, Mean(pg/ml): 230, Standard deviation: 17.02, CV(%): 7.4
• Sample 2: n=24, Mean(pg/ml): 645, Standard deviation: 39.99, CV(%): 6.2
• Sample 3: n=24, Mean(pg/ml): 1742, Standard deviation: 113.23, CV(%): 6.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Autre désignation: PI3 (PI3 Produits)

Synonymes: ESI Kit ELISA, SKALP Kit ELISA, WAP3 Kit ELISA, WFDC14 Kit ELISA, cementoin Kit ELISA, trappin-2 Kit ELISA, TRAPPIN-2 Kit ELISA, elafin Kit ELISA, peptidase inhibitor 3 Kit ELISA, peptidase inhibitor 3, skin-derived (SKALP) Kit ELISA, PI3 Kit ELISA

Sujet:

Protein Function: Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

Background: Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase(SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core(WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

Synonyms: Elafin,Elastase-specific inhibitor,ESI,Peptidase inhibitor 3,PI-3,Protease inhibitor WAP3,Skin-derived antileukoproteinase,SKALP,WAP four-disulfide core domain protein 14,PI3,WAP3, WFDC14,

Full Gene Name: Elafin

Cellular Localisation: Secreted.

ID gène: 5266

UniProt: P19957