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CCL16 Kit ELISA

CCL16 Reactivité: Humain AA 24-120 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1706078
  • Antigène Voir toutes CCL16 Kits ELISA
    CCL16 (Chemokine (C-C Motif) Ligand 16 (CCL16))
    Épitope
    AA 24-120
    Reactivité
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    7.8-500 pg/mL
    Seuil minimal de détection
    7.8 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL16/HCC-4
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: E.coli
    Immunogen sequence: Q24-Q120
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: E.coli
    Immunogen sequence: Q24-Q120
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

    Tissue Specificity: Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma- activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.

    Plaque
    Pre-coated
    Protocole
    human CCL16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL16 has been precoated onto 96-well plates. Standards(E.coli, Q24-Q120) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL16 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human CCL16 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human CCL16 standard solution and each sample is measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 25, Standard deviation: 0.80, CV(%): 3.2
    • Sample 2: n=16, Mean(pg/ml): 76, Standard deviation: 4.26, CV(%): 5.6
    • Sample 3: n=16, Mean(pg/ml): 237, Standard deviation: 13.98, CV(%): 5.9,
    • Sample 1: n=24, Mean(pg/ml): 26, Standard deviation: 1.51, CV(%): 5.8
    • Sample 2: n=24, Mean(pg/ml): 81, Standard deviation: 5.02, CV(%): 6.2
    • Sample 3: n=24, Mean(pg/ml): 242, Standard deviation: 16.21, CV(%): 6.7
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes CCL16 Kits ELISA
    CCL16 (Chemokine (C-C Motif) Ligand 16 (CCL16))
    Autre désignation
    CCL16 (CCL16 Produits)
    Synonymes
    CKb12 Kit ELISA, HCC-4 Kit ELISA, ILINCK Kit ELISA, LCC-1 Kit ELISA, LEC Kit ELISA, LMC Kit ELISA, Mtn-1 Kit ELISA, NCC-4 Kit ELISA, NCC4 Kit ELISA, SCYA16 Kit ELISA, SCYL4 Kit ELISA, C-C motif chemokine ligand 16 Kit ELISA, CCL16 Kit ELISA, Ccl16 Kit ELISA
    Sujet

    Protein Function: Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES.

    Background: Chemokine(C-C motif) ligand 16(CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Liver-expressed chemokine(LEC) and Monotactin-1(MTN-1). This chemokine is expressed by the liver, thymus, and spleen and is chemoattractive for monocytes and lymphocytes. Cellular expression of CCL16 can be strongly induced in monocytes by IL-10, IFN-gamma and bacterial lipopolysaccharide. Its gene is located on chromosome 17, in humans, among a cluster of other CC chemokines. CCL16 elicits its effects on cells by interacting with cell surface chemokine receptors such as CCR1, CCR2, CCR5 and CCR8.

    Synonyms: C-C motif chemokine 16,Chemokine CC-4,HCC-4,Chemokine LEC,IL-10-inducible chemokine,LCC-1,Liver-expressed chemokine,Lymphocyte and monocyte chemoattractant,LMC,Monotactin-1,MTN-1,NCC-4,Small-inducible cytokine A16,CCL16,ILINCK, NCC4, SCYA16,

    Full Gene Name: C-C motif chemokine 16

    Cellular Localisation: Secreted.
    ID gène
    6360
    UniProt
    O15467
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