GDF15 Kit ELISA

N° du produit ABIN1706082

Détail du produit GDF15 Kit ELISA

Antigène: GDF15 (Growth Differentiation Factor 15 (GDF15))

Épitope: AA 189-303

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse GDF-15

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: S189-A303

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: S189-A303

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Highly expressed in liver. .

Plaque: Pre-coated

Protocole: mouse GDF-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for GDF-15 has been precoated onto 96-well plates. Standards(NSO, S189-A303) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDF-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse GDF-15 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse GDF-15 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse GDF-15 standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 114, Standard deviation: 4.33, CV(%): 3.8
• Sample 2: n=16, Mean(pg/ml): 346, Standard deviation: 12.46, CV(%): 3.6
• Sample 3: n=16, Mean(pg/ml): 548, Standard deviation: 22.47, CV(%): 4.1,
• Sample 1: n=24, Mean(pg/ml): 98.2, Standard deviation: 7.37, CV(%): 7.5
• Sample 2: n=24, Mean(pg/ml): 359, Standard deviation: 20.10, CV(%): 5.6
• Sample 3: n=24, Mean(pg/ml): 579, Standard deviation: 39.37, CV(%): 6.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: GDF15 (Growth Differentiation Factor 15 (GDF15))

Autre désignation: GDF15 (GDF15 Produits)

Synonymes: GDF15 Kit ELISA, GDF-15 Kit ELISA, MIC-1 Kit ELISA, MIC1 Kit ELISA, NAG-1 Kit ELISA, PDF Kit ELISA, PLAB Kit ELISA, PTGFB Kit ELISA, SBF Kit ELISA, growth differentiation factor 15 Kit ELISA, GDF15 Kit ELISA, Gdf15 Kit ELISA

Sujet:

Background: GDF-15(Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.

Synonyms: Growth/differentiation factor 15,GDF-15,Gdf15,Sbf,

Full Gene Name: Growth/differentiation factor 15

Cellular Localisation: Secreted.

ID gène: 23886

UniProt: Q9Z0J7

Pathways: SARS-CoV-2 Protein Interactome