CCL8 Kit ELISA

N° du produit ABIN1706084

Détail du produit CCL8 Kit ELISA

Antigène: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Épitope: AA 20-97

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL8/MCP2

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: E20-P97

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: E20-P97

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse CCL8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL8 has been precoated onto 96-well plates. Standards(E.coli, E20-P97) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL8 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse CCL8 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse CCL8 standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 32, Standard deviation: 1.31, CV(%): 4.1
• Sample 2: n=16, Mean(pg/ml): 125, Standard deviation: 5.88, CV(%): 4.7
• Sample 3: n=16, Mean(pg/ml): 524, Standard deviation: 27.25, CV(%): 5.2,
• Sample 1: n=24, Mean(pg/ml): 33, Standard deviation: 1.68, CV(%): 5.1
• Sample 2: n=24, Mean(pg/ml): 129, Standard deviation: 8.39, CV(%): 6.5
• Sample 3: n=24, Mean(pg/ml): 531, Standard deviation: 35.58, CV(%): 6.7

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Autre désignation: CCL8 (CCL8 Produits)

Synonymes: HC14 Kit ELISA, MCP-2 Kit ELISA, MCP2 Kit ELISA, SCYA10 Kit ELISA, SCYA8 Kit ELISA, 1810063B20Rik Kit ELISA, AB023418 Kit ELISA, Mcp2 Kit ELISA, Scya8 Kit ELISA, C-C motif chemokine ligand 8 Kit ELISA, chemokine (C-C motif) ligand 8 Kit ELISA, C-C motif chemokine 8 Kit ELISA, monocyte chemoattractant protein-2 precursor Kit ELISA, CCL8 Kit ELISA, Ccl8 Kit ELISA, LOC101119572 Kit ELISA, MCP-2 Kit ELISA

Sujet:

Protein Function: Chemotactic factor that attracts monocytes. This protein can bind heparin (By similarity). .

Background: Chemokine(C-C motif) ligand 8, also known as monocyte chemoattractant protein 2(MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2(MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.

Synonyms: C-C motif chemokine 8,Monocyte chemoattractant protein 2,Monocyte chemotactic protein 2,MCP-2,Small-inducible cytokine A8,Ccl8,Mcp2, Scya8,

Full Gene Name: C-C motif chemokine 8

Cellular Localisation: Secreted.

ID gène: 20307

UniProt: Q9Z121