SensoLyte® pNPP Protein Phosphatase Assay Kit

N° du produit ABIN1882545

Détail du produit

Antigène: pNPP (PHOP) (Alkaline Phosphatase Synthesis Transcriptional Regulatory Protein (PHOP))

Méthode de détection: Colorimetric

Application: Enzyme Activity Assay (EAA)

Marque: SensoLyte®

Attributs du produit: SensoLyte® pNPP Protein Phosphatase Assay Kit uses pNNP to quantify protein phosphatase activities. The kit can be used for characterizing kinetics of enzyme reaction and high throughput screening of protein phosphatase inhibitors. It has high sensitivity and wide linear range (The detection limit is generally 3 ng or below). The kit contains:
pNNP chromogenic substrate
Protein phosphatase inhibitor
Assay buffer
A 'mix and read' assay protocol that is compatible with HTS liquid handling instruments

Information d'application

Commentaires:

Enzymatic Activity Assay kit

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Store kit components at -20 °C. Components B, C, D, and E can be stored at room temperature for convenience.

Référence

Rebollo, Roglans, Baena, Sánchez, Merlos, Alegret, Laguna: "Liquid fructose downregulates Sirt1 expression and activity and impairs the oxidation of fatty acids in rat and human liver cells." dans: Biochimica et biophysica acta, Vol. 1841, Issue 4, pp. 514-24, (2014) (PubMed).

Linford, Jiang, Edwards, Sherman, Van Voorhis, Stewart, Myler, Staker, Petri: "Crystal structure and putative substrate identification for the Entamoeba histolytica low molecular weight tyrosine phosphatase." dans: Molecular and biochemical parasitology, Vol. 193, Issue 1, pp. 33-44, (2014) (PubMed).

Lin, Ho, Cheng, You, Cheng, Yu, Chiu: "Impaired dephosphorylation renders G6PD-knockdown HepG2 cells more susceptible to H(2)O(2)-induced apoptosis." dans: Free radical biology & medicine, Vol. 49, Issue 3, pp. 361-73, (2010) (PubMed).

Svensson, Boren, Sköld, Flth, Sjögren, Andersson, Svenningsson, Andren: "Heat stabilization of the tissue proteome: a new technology for improved proteomics." dans: Journal of proteome research, Vol. 8, Issue 2, pp. 974-81, (2009) (PubMed).

Cho, Oh, Zhu: "Tyrosine phosphatase SHP-1 in oxidative stress and development of allergic airway inflammation." dans: American journal of respiratory cell and molecular biology, Vol. 39, Issue 4, pp. 412-9, (2008) (PubMed).

Coyne, Kim, Bergelson: "Poliovirus entry into human brain microvascular cells requires receptor-induced activation of SHP-2." dans: The EMBO journal, Vol. 26, Issue 17, pp. 4016-28, (2007) (PubMed).

Détail du antigène

Antigène: pNPP (PHOP) (Alkaline Phosphatase Synthesis Transcriptional Regulatory Protein (PHOP))

Autre désignation: pNPP

Sujet: Protein phosphatases have received great attentions as drug-screening targets. p-Nitrophenyl phosphate (pNPP) is proven to be an effective chromogenic substrate for protein tyrosine phosphatases and serine/threonine phosphatases.