SensoLyte® 520 Thrombin Activity Assay Kit

N° du produit ABIN1882567

Détail du produit

Antigène: alpha-Thrombin

Méthode de détection: Fluorometric

Application: Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)

Marque: SensoLyte®

Attributs du produit: The SensoLyte® 520 Thrombin Assay Kit is optimized for detecting thrombin activity. This kit contains a novel internally quenched 5-FAM/QXL™ 520 FRET substrate for thrombin. Thrombin cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence which can be monitored at excitation/emission= 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can be performed in a convenient 96-well or 384-well microplate format for screening of enzyme inhibitors.

Information d'application

Commentaires:

FRET-based Assay Kit

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Aliquot as needed to avoid multiple freeze-thaw cycles. Protect Components A and B from light and moisture.

Stock: -80 °C

Stockage commentaire: Store all kit components, except Component D, at -20 °C. Store Component D at -80 °C. Components C and F can be stored at room temperature for convenience.

Référence

Aleman-Garcia, Orbach, Willner: "Ion-responsive hemin-G-quadruplexes for switchable DNAzyme and enzyme functions." dans: Chemistry (Weinheim an der Bergstrasse, Germany), Vol. 20, Issue 19, pp. 5619-24, (2014) (PubMed).

Mogami, Keller, Shi, Word: "Effect of thrombin on human amnion mesenchymal cells, mouse fetal membranes, and preterm birth." dans: The Journal of biological chemistry, Vol. 289, Issue 19, pp. 13295-307, (2014) (PubMed).

Zheng, Wang, Liu, Pei, Huang, Wang: "System-level multi-target drug discovery from natural products with applications to cardiovascular diseases." dans: Molecular diversity, Vol. 18, Issue 3, pp. 621-35, (2014) (PubMed).

Gugerell, Schossleitner, Wolbank, Nürnberger, Redl, Gulle, Goppelt, Bittner, Pasteiner: "High thrombin concentrations in fibrin sealants induce apoptosis in human keratinocytes." dans: Journal of biomedical materials research. Part A, Vol. 100, Issue 5, pp. 1239-47, (2012) (PubMed).

Martini, Hammerer-Lercher, Zuck, Jekle, Debabov, Anderson, Nagl: "Antimicrobial and anticoagulant activities of N-chlorotaurine, N,N-dichloro-2,2-dimethyltaurine, and N-monochloro-2,2-dimethyltaurine in human blood." dans: Antimicrobial agents and chemotherapy, Vol. 56, Issue 4, pp. 1979-84, (2012) (PubMed).

Chen, Teramura, Iwata: "Immobilization of anticoagulant-loaded liposomes on cell surfaces by DNA hybridization." dans: Biomaterials, Vol. 32, Issue 31, pp. 7971-7, (2011) (PubMed).

Luan, Teramura, Iwata: "Layer-by-layer co-immobilization of soluble complement receptor 1 and heparin on islets." dans: Biomaterials, Vol. 32, Issue 27, pp. 6487-92, (2011) (PubMed).

Détail du antigène

Antigène: alpha-Thrombin

Autre désignation: Thrombin (alpha-Thrombin Produits)

Synonymes: PT Kit, RPRGL2 Kit, THPH1 Kit, coagulation factor II, thrombin Kit, F2 Kit

Sujet: Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation. Thrombin also influences a number of normal and pathological processes, including inflammation, tissue repair, embryogenesis, angiogenesis, and tumor invasion.