IGFBP2 Kit ELISA

N° du produit ABIN1889295

Détail du produit IGFBP2 Kit ELISA

Antigène: IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))

Épitope: AA 35-305

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 125-8000 pg/mL

Seuil minimal de détection: 125 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IGFBP2

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: E35-Q305

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: E35-Q305

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 1 IGFBP N-terminal domain.

Tissue Specificity: Highly expressed in adult liver, but also in kidney, lung, brain, spleen, testis and ovary. .

Plaque: Pre-coated

Protocole: mouse IGFBP2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP2 has been precoated onto 96-well plates. Standards(NSO, E35-Q305) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGFBP2 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse IGFBP2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IGFBP2 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 1257, Standard deviation: 89.2, CV(%): 7.1
• Sample 2: n=16, Mean(pg/ml): 3204, Standard deviation: 166.6, CV(%): 5.2
• Sample 3: n=16, Mean(pg/ml): 5394, Standard deviation: 350.61, CV(%): 6.5,
• Sample 1: n=24, Mean(pg/ml): 1020, Standard deviation: 84.66, CV(%): 8.3
• Sample 2: n=24, Mean(pg/ml): 3562, Standard deviation: 239, CV(%): 6.7
• Sample 3: n=24, Mean(pg/ml): 4975, Standard deviation: 368.15, CV(%): 7.4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))

Autre désignation: IGFBP2 (IGFBP2 Produits)

Synonymes: IBP2 Kit ELISA, IGF-BP53 Kit ELISA, AI255832 Kit ELISA, IBP-2 Kit ELISA, Igfbp-2 Kit ELISA, mIGFBP-2 Kit ELISA, IGFBP-2 Kit ELISA, ILGFBPA Kit ELISA, pIGFBP-2 Kit ELISA, igfbp2 Kit ELISA, MGC65741 Kit ELISA, MGC76823 Kit ELISA, MGC174445 Kit ELISA, IGFBP2 Kit ELISA, ibp2 Kit ELISA, igf-bp53 Kit ELISA, igfbp2a Kit ELISA, si:ch211-2k18.3 Kit ELISA, insulin like growth factor binding protein 2 Kit ELISA, insulin-like growth factor binding protein 2 Kit ELISA, insulin-like growth factor binding protein 2a Kit ELISA, insulin-like growth factor binding protein 2b Kit ELISA, IGFBP2 Kit ELISA, Igfbp2 Kit ELISA, igfbp2a Kit ELISA, igfbp2 Kit ELISA, igfbp2b Kit ELISA

Sujet:

Protein Function: Inhibits IGF-mediated growth and developmental rates (By similarity). IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. .

Background: Insulin-like growth factor-binding protein 2(IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.

Synonyms: Insulin-like growth factor-binding protein 2,IBP-2,IGF-binding protein 2,IGFBP-2,mIGFBP-2,Igfbp2,Igfbp-2,

Full Gene Name: Insulin-like growth factor-binding protein 2

Cellular Localisation: Secreted.

ID gène: 16008

UniProt: P47877

Pathways: Myometrial Relaxation and Contraction, Growth Factor Binding, Activated T Cell Proliferation