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CD36 Kit ELISA

CD36 Reactivité: Humain AA 30-439 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1889313
  • Antigène Voir toutes CD36 Kits ELISA
    CD36
    Épitope
    AA 30-439
    Reactivité
    • 7
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156-10000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD36/SR-B3
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: sf21
    Immunogen sequence: G30-N439
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: sf21
    Immunogen sequence: G30-N439
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the CD36 family.

    Plaque
    Pre-coated
    Protocole
    human CD36 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD36 has been precoated onto 96-well plates. Standards(sf21, G30-N439) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD36 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD36 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CD36 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD36 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 1.26, Standard deviation: 0.049, CV(%): 3.9
    • Sample 2: n=16, Mean(ng/ml): 4.31, Standard deviation: 0.310, CV(%): 7.2
    • Sample 3: n=16, Mean(ng/ml): 6.28, Standard deviation: 0.427, CV(%): 6.8,
    • Sample 1: n=24, Mean(ng/ml): 1.33, Standard deviation: 0.061, CV(%): 4.6
    • Sample 2: n=24, Mean(ng/ml): 4.56, Standard deviation: 0.374, CV(%): 8.2
    • Sample 3: n=24, Mean(ng/ml): 7.24, Standard deviation: 0.565, CV(%): 7.8
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes CD36 Kits ELISA
    CD36
    Autre désignation
    CD36 (CD36 Produits)
    Synonymes
    BDPLT10 Kit ELISA, CHDS7 Kit ELISA, FAT Kit ELISA, GP3B Kit ELISA, GP4 Kit ELISA, GPIV Kit ELISA, PASIV Kit ELISA, SCARB3 Kit ELISA, Fat Kit ELISA, Scarb3 Kit ELISA, GPIIIB Kit ELISA, PAS-4 Kit ELISA, zgc:92513 Kit ELISA, CD36 molecule Kit ELISA, CD36 molecule (thrombospondin receptor) Kit ELISA, CD36 Kit ELISA, Cd36 Kit ELISA, cd36 Kit ELISA
    Sujet

    Protein Function: Binds to collagen, thrombospondin, anionic phospholipids and oxidized low-density lipoprotein (oxLDL). May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects. As a coreceptor for TLR4-TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, rapidly induces the formation of a heterodimer of TLR4 and TLR6, which is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. .

    Background: CD36(Cluster of Differentiation 36) is an integral membrane protein found on the surface of many cell types in vertebrate animals and is also known as FAT, SCARB3, GP88, glycoprotein IV(gpIV) and glycoprotein IIIb(gpIIIb). The human CD36 is a member of a gene family of structurally related glycoproteins and functions as a receptor for collagen type I and thrombospondin. The use of a CD36 genomic probe has allowed the localization of the CD36 locus to the 7q11.2 band by fluorescence in situ hybridization coupled with GTG-banding. CED-1/SCARF1 and C03F11.3/CD36 are beta-glucan binding receptors and define an evolutionarily conserved pathway for the innate sensing of fungal pathogens.

    Synonyms: Platelet glycoprotein 4,Fatty acid translocase,FAT,Glycoprotein IIIb,GPIIIB,Leukocyte differentiation antigen CD36,PAS IV,PAS-4,Platelet collagen receptor,Platelet glycoprotein IV,GPIV,Thrombospondin receptor,CD36,CD36,GP3B, GP4,

    Full Gene Name: Platelet glycoprotein 4

    Cellular Localisation: Cell membrane, Multi-pass membrane protein. Upon ligand-binding, internalized through dynamin-dependent endocytosis..
    ID gène
    948
    UniProt
    P16671
    Pathways
    Signalisation TLR, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism, S100 Proteins
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