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CXCL7 Kit ELISA

PPBP Reactivité: Humain AA 59-128 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1889316
  • Antigène Voir toutes CXCL7 (PPBP) Kits ELISA
    CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))
    Épitope
    AA 59-128
    Reactivité
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.6-1000 pg/mL
    Seuil minimal de détection
    15.6 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL7
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: E.coli
    Immunogen sequence: A59-D128
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <2pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: E.coli
    Immunogen sequence: A59-D128
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

    Plaque
    Pre-coated
    Protocole
    human CXCL7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL7 has been precoated onto 96-well plates. Standards(E.coli, A59-D128) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL7 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CXCL7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL7 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 85.6, Standard deviation: 3.85, CV(%): 4.5
    • Sample 2: n=16, Mean(pg/ml): 227, Standard deviation: 12.7, CV(%): 5.6
    • Sample 3: n=16, Mean(pg/ml): 519, Standard deviation: 30.1, CV(%): 5.8,
    • Sample 1: n=24, Mean(pg/ml): 82.8, Standard deviation: 6.13, CV(%): 7.4
    • Sample 2: n=24, Mean(pg/ml): 236, Standard deviation: 14.6, CV(%): 6.2
    • Sample 3: n=24, Mean(pg/ml): 527, Standard deviation: 37.4, CV(%): 7.1
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes CXCL7 (PPBP) Kits ELISA
    CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))
    Autre désignation
    CXCL7 (PPBP Produits)
    Synonymes
    2400003M24Rik Kit ELISA, AI854500 Kit ELISA, CTAP3 Kit ELISA, CTAPIII Kit ELISA, Cxcl7 Kit ELISA, LA-PF4 Kit ELISA, LDGF Kit ELISA, MDGF Kit ELISA, NAP-2 Kit ELISA, NAP-2-L1 Kit ELISA, Scyb7 Kit ELISA, TGB Kit ELISA, TGB1 Kit ELISA, THBGB1 Kit ELISA, b-TG1 Kit ELISA, beta-TG Kit ELISA, B-TG1 Kit ELISA, Beta-TG Kit ELISA, CTAP-III Kit ELISA, CXCL7 Kit ELISA, PBP Kit ELISA, SCYB7 Kit ELISA, TC1 Kit ELISA, TC2 Kit ELISA, THBGB Kit ELISA, Nap-2 Kit ELISA, CTAPIII/NAP-2 Kit ELISA, Platelet basic protein Kit ELISA, pro-platelet basic protein Kit ELISA, scyb7 Kit ELISA, Ppbp Kit ELISA, PPBP Kit ELISA
    Sujet

    Protein Function: LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation. .

    Background: Chemokine(C-X-C motif) ligand 7(CXCL7), also called PPBP or SCYB7, is a human gene. The encoded protein, Chemokine(C-X-C motif) ligand is a small cytokine belonging to the CXC chemokine family. CXCL7 is mapped to 4q13.3. It is a protein that is released in large amounts from platelets following their activation. CXCL7 is the precursor of the 2 platelet alpha-granule proteins, platelet basic protein(PBP) and connective tissue-activating peptide III(CTAP3). It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

    Synonyms: Platelet basic protein,PBP,C-X-C motif chemokine 7,Leukocyte-derived growth factor,LDGF,Macrophage-derived growth factor,MDGF,Small-inducible cytokine B7,Connective tissue-activating peptide III,CTAP-III,LA-PF4,Low-affinity platelet factor IV,TC-2,Connective tissue-activating peptide III(1-81),CTAP-III(1-81),Beta-thromboglobulin,Beta-TG,Neutrophil-activating peptide 2(74),NAP-2(74),Neutrophil-activating peptide 2(73),NAP-2(73),Neutrophil-activating peptide 2,NAP-2,TC-1,Neutrophil-activating peptide 2(1-66),NAP-2(1-66),Neutrophil-activating peptide 2(1-63),NAP-2(1-63),PPBP,CTAP3, CXCL7, SCYB7, TGB1, THBGB1,

    Full Gene Name: Platelet basic protein

    Cellular Localisation: Secreted.
    ID gène
    5473
    UniProt
    P02775
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