CXCL7 Kit ELISA

N° du produit ABIN1889316

Détail du produit CXCL7 Kit ELISA

Antigène: CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))

Épitope: AA 59-128

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL7

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: A59-D128

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <2pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: A59-D128

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

Plaque: Pre-coated

Protocole: human CXCL7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL7 has been precoated onto 96-well plates. Standards(E.coli, A59-D128) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL7 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CXCL7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL7 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 85.6, Standard deviation: 3.85, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 227, Standard deviation: 12.7, CV(%): 5.6
• Sample 3: n=16, Mean(pg/ml): 519, Standard deviation: 30.1, CV(%): 5.8,
• Sample 1: n=24, Mean(pg/ml): 82.8, Standard deviation: 6.13, CV(%): 7.4
• Sample 2: n=24, Mean(pg/ml): 236, Standard deviation: 14.6, CV(%): 6.2
• Sample 3: n=24, Mean(pg/ml): 527, Standard deviation: 37.4, CV(%): 7.1

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))

Autre désignation: CXCL7 (PPBP Produits)

Synonymes: 2400003M24Rik Kit ELISA, AI854500 Kit ELISA, CTAP3 Kit ELISA, CTAPIII Kit ELISA, Cxcl7 Kit ELISA, LA-PF4 Kit ELISA, LDGF Kit ELISA, MDGF Kit ELISA, NAP-2 Kit ELISA, NAP-2-L1 Kit ELISA, Scyb7 Kit ELISA, TGB Kit ELISA, TGB1 Kit ELISA, THBGB1 Kit ELISA, b-TG1 Kit ELISA, beta-TG Kit ELISA, B-TG1 Kit ELISA, Beta-TG Kit ELISA, CTAP-III Kit ELISA, CXCL7 Kit ELISA, PBP Kit ELISA, SCYB7 Kit ELISA, TC1 Kit ELISA, TC2 Kit ELISA, THBGB Kit ELISA, Nap-2 Kit ELISA, CTAPIII/NAP-2 Kit ELISA, Platelet basic protein Kit ELISA, pro-platelet basic protein Kit ELISA, scyb7 Kit ELISA, Ppbp Kit ELISA, PPBP Kit ELISA

Sujet:

Protein Function: LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation. .

Background: Chemokine(C-X-C motif) ligand 7(CXCL7), also called PPBP or SCYB7, is a human gene. The encoded protein, Chemokine(C-X-C motif) ligand is a small cytokine belonging to the CXC chemokine family. CXCL7 is mapped to 4q13.3. It is a protein that is released in large amounts from platelets following their activation. CXCL7 is the precursor of the 2 platelet alpha-granule proteins, platelet basic protein(PBP) and connective tissue-activating peptide III(CTAP3). It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

Synonyms: Platelet basic protein,PBP,C-X-C motif chemokine 7,Leukocyte-derived growth factor,LDGF,Macrophage-derived growth factor,MDGF,Small-inducible cytokine B7,Connective tissue-activating peptide III,CTAP-III,LA-PF4,Low-affinity platelet factor IV,TC-2,Connective tissue-activating peptide III(1-81),CTAP-III(1-81),Beta-thromboglobulin,Beta-TG,Neutrophil-activating peptide 2(74),NAP-2(74),Neutrophil-activating peptide 2(73),NAP-2(73),Neutrophil-activating peptide 2,NAP-2,TC-1,Neutrophil-activating peptide 2(1-66),NAP-2(1-66),Neutrophil-activating peptide 2(1-63),NAP-2(1-63),PPBP,CTAP3, CXCL7, SCYB7, TGB1, THBGB1,

Full Gene Name: Platelet basic protein

Cellular Localisation: Secreted.

ID gène: 5473

UniProt: P02775