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XCL1 Kit ELISA

XCL1 Reactivité: Humain AA 22-114 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1889320
  • Antigène Voir toutes XCL1 Kits ELISA
    XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))
    Épitope
    AA 22-114
    Reactivité
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    62.5-4000 pg/mL
    Seuil minimal de détection
    62.5 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human XCL1/Lymphotactin
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: E.coli
    Immunogen sequence: V22-G114
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <1pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: E.coli
    Immunogen sequence: V22-G114
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the intercrine gamma family.

    Tissue Specificity: Highest level in spleen, lower in peripheral leukocytes and very low levels in lung, colon and small intestine.

    Plaque
    Pre-coated
    Protocole
    human XCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for XCL1 has been precoated onto 96-well plates. Standards(E.coli, V22-G114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for XCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human XCL1 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human XCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human XCL1 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 1.08, Standard deviation: 0.045, CV(%): 4.2
    • Sample 2: n=16, Mean(ng/ml): 2.12, Standard deviation: 0.076, CV(%): 3.6
    • Sample 3: n=16, Mean(ng/ml): 3.85, Standard deviation: 0.2, CV(%): 5.2,
    • Sample 1: n=24, Mean(ng/ml): 1.42, Standard deviation: 0.097, CV(%): 6.8
    • Sample 2: n=24, Mean(ng/ml): 2.56, Standard deviation: 0.184, CV(%): 7.2
    • Sample 3: n=24, Mean(ng/ml): 3.78, Standard deviation: 0.295, CV(%): 7.8
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes XCL1 Kits ELISA
    XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))
    Autre désignation
    XCL1 (XCL1 Produits)
    Synonymes
    ATAC Kit ELISA, LPTN Kit ELISA, LTN Kit ELISA, SCM-1 Kit ELISA, SCM-1a Kit ELISA, SCM1 Kit ELISA, SCM1A Kit ELISA, SCYC1 Kit ELISA, AI661682 Kit ELISA, Lptn Kit ELISA, Scyc1 Kit ELISA, Ltn Kit ELISA, XCL1 Kit ELISA, XCL2 Kit ELISA, X-C motif chemokine ligand 1 Kit ELISA, chemokine (C motif) ligand 1 Kit ELISA, cytokine SCM-1 beta Kit ELISA, lymphotactin Kit ELISA, XCL1 Kit ELISA, Xcl1 Kit ELISA, LOC490356 Kit ELISA, LOC100714756 Kit ELISA, LOC100349255 Kit ELISA
    Sujet

    Protein Function: Chemotactic activity for lymphocytes but not for monocytes or neutrophils.

    Background: Chemokine(C motif) ligand(XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. This gene is mapped to chromosome 1q23 by FISH. The sequence of the deduced 114-amino acid protein is most homologous to the CC chemokines CCL8 and CCL3. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces the migration of cells expressing XCR1.

    Synonyms: Lymphotactin,ATAC,C motif chemokine 1,Cytokine SCM-1,Lymphotaxin,SCM-1-alpha,Small-inducible cytokine C1,XC chemokine ligand 1,XCL1,LTN, SCYC1,

    Full Gene Name: Lymphotactin

    Cellular Localisation: Secreted.
    ID gène
    6375
    UniProt
    P47992
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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