Kallikrein 6 Kit ELISA

N° du produit ABIN1889322

Détail du produit Kallikrein 6 Kit ELISA

Antigène: Kallikrein 6 (KLK6)

Épitope: AA 22-244

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78-5000 pg/mL

Seuil minimal de détection: 78 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Kallikrein-6

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: L22-K244

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <3pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: L22-K244

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the peptidase S1 family. Kallikrein subfamily.

Tissue Specificity: In fluids, highest levels found in milk of lactating women followed by cerebrospinal fluid, nipple aspirate fluid and breast cyst fluid. Also found in serum, seminal plasma and some amniotic fluids and breast tumor cytosolic extracts. Not detected in urine. At the tissue level, highest concentrations found in glandular tissues such as salivary glands followed by lung, colon, fallopian tube, placenta, breast, pituitary and kidney. Not detected in skin, spleen, bone, thyroid, heart, ureter, liver, muscle, endometrium, testis, pancreas, seminal vesicle, ovary, adrenals and prostate. In brain, detected in gray matter neurons (at protein level). Colocalizes with pathological inclusions such as Lewy bodies and glial cytoplasmic inclusions. Overexpressed in primary breast tumors but not expressed in metastatic tumors. .

Plaque: Pre-coated

Protocole: human Kallikrein-6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kallikrein-6 has been precoated onto 96-well plates. Standards(NSO, L22-K244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kallikrein-6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Kallikrein-6 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human Kallikrein-6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Kallikrein-6 standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 545, Standard deviation: 37.61, CV(%): 6.9
• Sample 2: n=16, Mean(pg/ml): 1711, Standard deviation: 80.42, CV(%): 4.7
• Sample 3: n=16, Mean(pg/ml): 2903, Standard deviation: 168.4, CV(%): 5.8,
• Sample 1: n=24, Mean(pg/ml): 721, Standard deviation: 55.52, CV(%): 7.7
• Sample 2: n=24, Mean(pg/ml): 1927, Standard deviation: 104.1, CV(%): 5.4
• Sample 3: n=24, Mean(pg/ml): 3120, Standard deviation: 196.6, CV(%): 6.3

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour Kallikrein 6 Kit ELISA (ABIN1889322)

Sells, Pandey, Chen, Skovan, Cui, Ignatenko: "Specific microRNA-mRNA Regulatory Network of Colon Cancer Invasion Mediated by Tissue Kallikrein-Related Peptidase 6." dans: Neoplasia (New York, N.Y.), Vol. 19, Issue 5, pp. 396-411, (2018) (PubMed).

Détail du antigène

Antigène: Kallikrein 6 (KLK6)

Autre désignation: KLK6 (KLK6 Produits)

Synonymes: Bssp Kit ELISA, Klk7 Kit ELISA, PRSS18 Kit ELISA, PRSS9 Kit ELISA, SP59 Kit ELISA, hK6 Kit ELISA, Prss9 Kit ELISA, AI849898 Kit ELISA, BSP Kit ELISA, Klk29 Kit ELISA, MSP Kit ELISA, Prss18 Kit ELISA, neurosin Kit ELISA, kallikrein related peptidase 6 Kit ELISA, kallikrein related-peptidase 6 Kit ELISA, kallikrein-6 Kit ELISA, KLK6 Kit ELISA, Klk6 Kit ELISA, LOC100716801 Kit ELISA

Sujet:

Protein Function: Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis. .

Background: KLK6(Kallikrein-related peptidase 6), also called KALLIKREIN 6, NEUROSIN, PROTEASE M, ZYME or PRSS9, is a protein that in humans is encoded by the KLK6 gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. Northern blot analysis revealed that the PRSS9 mRNA was expressed in several primary tumors and cell lines from mammary, prostate, and ovarian cancers, but was not detected in any metastases of these cancers. The KLK6 gene is mapped on 19q13.41. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease. Upon cellular stress, neurosin was released from mitochondria to the cytosol, which resulted in the increase of degraded alpha-synuclein species. Neurosin may play a significant role in physiologic alpha-synuclein degradation and also in the pathogenesis of synucleinopathies.

Synonyms: Kallikrein-6,3.4.21.-,Neurosin,Protease M,SP59,Serine protease 18,Serine protease 9,Zyme,KLK6,PRSS18, PRSS9,

Full Gene Name: Kallikrein-6

Cellular Localisation: Secreted. Nucleus, nucleolus. Cytoplasm. Mitochondrion. Microsome. In brain, detected in the nucleus of glial cells and in the nucleus and cytoplasm of neurons. Detected in the mitochondrial and microsomal fractions of HEK-293 cells and released into the cytoplasm following cell stress.

ID gène: 5653

UniProt: Q92876

Pathways: Système du Complément, Regulation of G-Protein Coupled Receptor Protein Signaling