TGFB2 Kit ELISA

N° du produit ABIN1889331

Détail du produit TGFB2 Kit ELISA

Antigène: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Épitope: AA 303-414

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Rat TGF-beta 2

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: A303-S414

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: A303-S414

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Isoform TGF-beta2B expressed in the aorta, primary bronchus, uterus, heart, skeletal muscle, sciatic nerve and spinal cord but not in the intestine. .

Plaque: Pre-coated

Protocole: rat TGF-beta 2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF-beta 2 has been precoated onto 96-well plates. Standards(NSO, A303-S414) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF-beta 2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TGF-beta 2 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat TGF-beta 2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of activated rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat TGF-beta 2 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 304, Standard deviation: 16.42, CV(%): 5.4
• Sample 2: n=16, Mean(pg/ml): 614, Standard deviation: 31.93, CV(%): 5.2
• Sample 3: n=16, Mean(pg/ml): 1172, Standard deviation: 79.7, CV(%): 6.8,
• Sample 1: n=24, Mean(pg/ml): 377, Standard deviation: 23.75, CV(%): 6.3
• Sample 2: n=24, Mean(pg/ml): 536, Standard deviation: 39.13, CV(%): 7.3
• Sample 3: n=24, Mean(pg/ml): 1223, Standard deviation: 88.1, CV(%): 7.2

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour TGFB2 Kit ELISA (ABIN1889331)

Li, Liu, Shen, Wang, Chen, Xu, Wen: "MicroRNA-26a modulates transforming growth factor beta-1-induced proliferation in human fetal lung fibroblasts." dans: Biochemical and biophysical research communications, Vol. 454, Issue 4, pp. 512-517, (2014) (PubMed).

Détail du antigène

Antigène: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Autre désignation: TGFB2 (TGFB2 Produits)

Synonymes: LDS4 Kit ELISA, TGF-beta2 Kit ELISA, tgf-beta2 Kit ELISA, tgfb2-A Kit ELISA, BB105277 Kit ELISA, Tgf-beta2 Kit ELISA, Tgfb-2 Kit ELISA, TGF-beta 2 Kit ELISA, Tgfbr2T Kit ELISA, TGFB2 Kit ELISA, TGFbeta2 Kit ELISA, MGF Kit ELISA, TGF-B2 Kit ELISA, transforming growth factor beta 2 Kit ELISA, transforming growth factor beta 2 L homeolog Kit ELISA, transforming growth factor, beta 2 Kit ELISA, transforming growth factor, beta receptor 2 Kit ELISA, TGFB2 Kit ELISA, tgfb2.L Kit ELISA, Tgfb2 Kit ELISA, Tgfbr2 Kit ELISA, tgfb2 Kit ELISA

Sujet:

Protein Function: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.

Background: Transforming growth factor-beta 2(TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma(POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured human anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.

Synonyms: Transforming growth factor beta-2,TGF-beta-2,Latency-associated peptide,LAP,Tgfb2,

Full Gene Name: Transforming growth factor beta-2

Cellular Localisation: Secreted.

ID gène: 81809

UniProt: Q07257

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus