TGFB2 Kit ELISA
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- Antigène Voir toutes TGFB2 Kits ELISA
- TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))
- Épitope
- AA 303-414
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 31.2-2000 pg/mL
- Seuil minimal de détection
- 31.2 pg/mL
- Application
- ELISA
- Fonction
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Mouse TGF-beta 2
- Marque
- PicoKine™
- Type d'échantillon
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
- Analytical Method
- Quantitative
- Specificité
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Expression system for standard: NSO
Immunogen sequence: A303-S414 - Réactivité croisée (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Sensibilité
- <10pg/mL
- Matériel non inclus
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- Immunogène
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Expression system for standard: NSO
Immunogen sequence: A303-S414 - Featured
- Discover our best selling TGFB2 Kit ELISA
- Top Product
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- Indications d'application
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- Plaque
- Pre-coated
- Protocole
- mouse TGF-beta 2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TGF-beta 2 has been precoated onto 96-well plates. Standards(NSO, A303-S414) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF-beta 2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TGF-beta 2 amount of sample captured in plate.
- Procédure de l'essai
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TGF-beta 2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of activated mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TGF-beta 2 standard solution and each sample be measured in duplicate.
- Précision du teste
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- Sample 1: n=16, Mean(pg/ml): 242, Standard deviation: 10.9, CV(%): 4.5
- Sample 2: n=16, Mean(pg/ml): 524, Standard deviation: 28.3, CV(%): 5.4
- Sample 3: n=16, Mean(pg/ml): 1096, Standard deviation: 73.43, CV(%): 6.7,
- Sample 1: n=24, Mean(pg/ml): 253, Standard deviation: 13, CV(%): 5.1
- Sample 2: n=24, Mean(pg/ml): 487, Standard deviation: 35.55, CV(%): 7.3
- Sample 3: n=24, Mean(pg/ml): 1295, Standard deviation: 90.65, CV(%): 7
- Restrictions
- For Research Use only
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- Conseil sur la manipulation
- Avoid multiple freeze-thaw cycles.
- Stock
- -20 °C,4 °C
- Stockage commentaire
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- Date de péremption
- 12 months
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MicroRNA-26a modulates transforming growth factor beta-1-induced proliferation in human fetal lung fibroblasts." dans: Biochemical and biophysical research communications, Vol. 454, Issue 4, pp. 512-517, (2014) (PubMed).
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MicroRNA-26a modulates transforming growth factor beta-1-induced proliferation in human fetal lung fibroblasts." dans: Biochemical and biophysical research communications, Vol. 454, Issue 4, pp. 512-517, (2014) (PubMed).
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- Antigène Voir toutes TGFB2 Kits ELISA
- TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))
- Autre désignation
- TGFB2 (TGFB2 Produits)
- Synonymes
- LDS4 Kit ELISA, TGF-beta2 Kit ELISA, tgf-beta2 Kit ELISA, tgfb2-A Kit ELISA, BB105277 Kit ELISA, Tgf-beta2 Kit ELISA, Tgfb-2 Kit ELISA, TGF-beta 2 Kit ELISA, Tgfbr2T Kit ELISA, TGFB2 Kit ELISA, TGFbeta2 Kit ELISA, MGF Kit ELISA, TGF-B2 Kit ELISA, transforming growth factor beta 2 Kit ELISA, transforming growth factor beta 2 L homeolog Kit ELISA, transforming growth factor, beta 2 Kit ELISA, transforming growth factor, beta receptor 2 Kit ELISA, TGFB2 Kit ELISA, tgfb2.L Kit ELISA, Tgfb2 Kit ELISA, Tgfbr2 Kit ELISA, tgfb2 Kit ELISA
- Sujet
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Protein Function: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.
Background: Transforming growth factor-beta 2(TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma(POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured human anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.
Synonyms: Transforming growth factor beta-2,TGF-beta-2,Latency-associated peptide,LAP,Tgfb2,
Full Gene Name: Transforming growth factor beta-2
Cellular Localisation: Secreted. - ID gène
- 21808
- UniProt
- P27090
- Pathways
- Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus
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