Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

TGFB3 Kit ELISA

TGFB3 Reactivité: Humain AA 301-412 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum, Urine
N° du produit ABIN1889334
  • Antigène Voir toutes TGFB3 Kits ELISA
    TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))
    Épitope
    AA 301-412
    Reactivité
    • 9
    • 8
    • 8
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2-2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Human TGF-beta 3
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (EDTA), Urine
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: sf21
    Immunogen sequence: A301-S412
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Homologie
    Bovine,Canine,Chicken,Horse,Monkey,Rabbit
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: sf21
    Immunogen sequence: A301-S412
    Featured
    Discover our best selling TGFB3 Kit ELISA
    Top Product
    Discover our top product TGFB3 Kit ELISA
  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the TGF-beta family.

    Plaque
    Pre-coated
    Protocole
    human TGF-beta 3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF-beta 3 has been precoated onto 96-well plates. Standards(sf21, A301-S412) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF-beta 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TGF-beta 3 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human TGF-beta 3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of activated human cell culture supernates, serum, plasma(EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TGF-beta 3 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 271, Standard deviation: 14.4, CV(%): 5.3
    • Sample 2: n=16, Mean(pg/ml): 631, Standard deviation: 29, CV(%): 4.6
    • Sample 3: n=16, Mean(pg/ml): 1153, Standard deviation: 45, CV(%): 3.9,
    • Sample 1: n=24, Mean(pg/ml): 294, Standard deviation: 18.82, CV(%): 6.4
    • Sample 2: n=24, Mean(pg/ml): 670, Standard deviation: 38.2, CV(%): 5.7
    • Sample 3: n=24, Mean(pg/ml): 1265, Standard deviation: 72.85, CV(%): 5.8
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes TGFB3 Kits ELISA
    TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))
    Autre désignation
    TGFB3 (TGFB3 Produits)
    Synonymes
    TGFB3 Kit ELISA, ARVD Kit ELISA, TGF-beta3 Kit ELISA, Tgfb-3 Kit ELISA, TGF-B3 Kit ELISA, TGFbeta3 Kit ELISA, wu:fc78g09 Kit ELISA, zgc:92058 Kit ELISA, transforming growth factor beta 3 Kit ELISA, transforming growth factor, beta 3 Kit ELISA, TGFB3 Kit ELISA, Tgfb3 Kit ELISA, tgfb3 Kit ELISA
    Sujet

    Protein Function: Involved in embryogenesis and cell differentiation.

    Background: Transforming growth factor beta 3(TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix(ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin(CTNNB1) via nuclear phospho-Smad2 and Smad4.

    Synonyms: Transforming growth factor beta-3,TGF-beta-3,Latency-associated peptide,LAP,TGFB3,

    Full Gene Name: Transforming growth factor beta-3

    Cellular Localisation: Secreted.
    ID gène
    7043
    UniProt
    P10600
    Pathways
    Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus
Vous êtes ici:
Support technique