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FGF19 Kit ELISA

FGF19 Reactivité: Humain AA 25-216 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1889347
  • Antigène Voir toutes FGF19 Kits ELISA
    FGF19 (Fibroblast Growth Factor 19 (FGF19))
    Épitope
    AA 25-216
    Reactivité
    • 8
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.6-1000 pg/mL
    Seuil minimal de détection
    15.6 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human FGF19
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: E.coli
    Immunogen sequence: L25-K216
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: E.coli
    Immunogen sequence: L25-K216
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: Expressed in fetal brain, cartilage, retina, and adult gall bladder. .

    Plaque
    Pre-coated
    Protocole
    human FGF19 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF19 has been precoated onto 96-well plates. Standards(E.coli, L25-K216) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF19 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FGF19 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human FGF19 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human FGF19 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 103, Standard deviation: 6.69, CV(%): 6.5
    • Sample 2: n=16, Mean(pg/ml): 396, Standard deviation: 17, CV(%): 4.3
    • Sample 3: n=16, Mean(pg/ml): 669, Standard deviation: 25.4, CV(%): 3.8,
    • Sample 1: n=24, Mean(pg/ml): 152, Standard deviation: 10.94, CV(%): 7.2
    • Sample 2: n=24, Mean(pg/ml): 388, Standard deviation: 20.95, CV(%): 5.4
    • Sample 3: n=24, Mean(pg/ml): 709, Standard deviation: 34.74, CV(%): 4.9
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes FGF19 Kits ELISA
    FGF19 (Fibroblast Growth Factor 19 (FGF19))
    Autre désignation
    FGF19 (FGF19 Produits)
    Synonymes
    FGF-19 Kit ELISA, Fgf15 Kit ELISA, fibroblast growth factor 19 Kit ELISA, FGF19 Kit ELISA, Fgf19 Kit ELISA, fgf19 Kit ELISA
    Sujet

    Protein Function: Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. .

    Background: FGF19, Fibroblast growth factor 19, is a protein that in humans is encoded by the FGF19 gene. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. The FGF19 gene is mapped to 11q13.3. The deduced 216-amino acid FGF19 protein contains asignal sequence and 2 cysteine residues that are conserved in the FGF family. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. FGF19 stimulates hepatic protein and glycogen synthesis but does not induce lipogenesis. The effects of FGF19 are independent of the activity of either insulin or the protein kinase Akt and, instead, are mediated through a mitogen-activated protein kinase signaling pathway that activates components of the protein translation machinery and stimulates glycogen synthase activity.

    Synonyms: Fibroblast growth factor 19,FGF-19,FGF19,UNQ334/PRO533,

    Full Gene Name: Fibroblast growth factor 19

    Cellular Localisation: Secreted.
    ID gène
    9965
    UniProt
    O95750
    Pathways
    Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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