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IL17RC Kit ELISA

IL17RC Reactivité: Souris AA 22-440 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1889355
  • Antigène Voir toutes IL17RC Kits ELISA
    IL17RC (Interleukin 17 Receptor C (IL17RC))
    Épitope
    AA 22-440
    Reactivité
    • 5
    • 3
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156-10.000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-17RC
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: E22-R440
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: E22-R440
  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plaque
    Pre-coated
    Protocole
    mouse IL-17RC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17RC has been precoated onto 96-well plates. Standards(NSO, E22-R440) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17RC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-17RC amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse IL-17RC standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-17RC standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 1458, Standard deviation: 61.24, CV(%): 4.2
    • Sample 2: n=16, Mean(pg/ml): 4112, Standard deviation: 267.3, CV(%): 6.5
    • Sample 3: n=16, Mean(pg/ml): 6822, Standard deviation: 388.9, CV(%): 5.7,
    • Sample 1: n=24, Mean(pg/ml): 1632, Standard deviation: 88.13, CV(%): 5.4
    • Sample 2: n=24, Mean(pg/ml): 4473, Standard deviation: 335.5, CV(%): 7.5
    • Sample 3: n=24, Mean(pg/ml): 7188, Standard deviation: 488.8, CV(%): 6.8
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes IL17RC Kits ELISA
    IL17RC (Interleukin 17 Receptor C (IL17RC))
    Autre désignation
    IL17RC (IL17RC Produits)
    Synonymes
    IL17-RL Kit ELISA, IL17RL Kit ELISA, 1110025H02Rik Kit ELISA, Gm19850 Kit ELISA, IL-17RC Kit ELISA, IL17-RC Kit ELISA, Il17rl Kit ELISA, interleukin 17 receptor C Kit ELISA, Il17rc Kit ELISA, IL17RC Kit ELISA
    Sujet

    Background: Interleukin-17 receptor C is a protein that in humans is encoded by the IL17RC gene. By genomic sequence analysis, IL17RC gene was mapped to chromosome 3p25.3-p24.1. This gene encodes a single-pass transmembrane protein that shares limited similarity with the interleukin-17 receptor. Multiple alternatively spliced transcript variants encoding different isoforms have been detected for this gene, but the full-length nature of only three have been determined to date. The biologic activity of IL-17 is dependent on a complex composed of IL-17RA and IL-17RC, suggesting a new paradigm for understanding the interactions between the expanded family of IL-17 ligands and their receptors.

    Synonyms: Interleukin-17 receptor C,IL-17 receptor C,IL-17RC,Interleukin-17 receptor-like protein,IL-17RL,ZcytoR14,Il17rc,

    Full Gene Name: Interleukin-17 receptor C

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    ID gène
    171095
    UniProt
    Q8K4C2
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