GAS6 Kit ELISA

N° du produit ABIN1889367

Détail du produit GAS6 Kit ELISA

Antigène: GAS6 (Growth Arrest-Specific 6 (GAS6))

Épitope: AA 115-673

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse GAS6

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: D115-P673

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: D115-P673

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 4 EGF-like domains.

Plaque: Pre-coated

Protocole: mouse GAS6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for GAS6 has been precoated onto 96-well plates. Standards(NSO, D115-P673) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GAS6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse GAS6 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse GAS6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse GAS6 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 156, Standard deviation: 9.048, CV(%): 5.8
• Sample 2: n=16, Mean(pg/ml): 724, Standard deviation: 34.03, CV(%): 4.7
• Sample 3: n=16, Mean(pg/ml): 1345, Standard deviation: 55.15, CV(%): 4.1,
• Sample 1: n=24, Mean(pg/ml): 174 , Standard deviation: 11.31, CV(%): 6.5
• Sample 2: n=24, Mean(pg/ml): 617, Standard deviation: 33.32, CV(%): 5.4
• Sample 3: n=24, Mean(pg/ml): 1436, Standard deviation: 112.01, CV(%): 7.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: GAS6 (Growth Arrest-Specific 6 (GAS6))

Autre désignation: GAS6 (GAS6 Produits)

Synonymes: fj34a12 Kit ELISA, zgc:63860 Kit ELISA, wu:fj34a12 Kit ELISA, MGC68463 Kit ELISA, gas6 Kit ELISA, MGC83872 Kit ELISA, GAS6 Kit ELISA, Gas-6 Kit ELISA, AXLLG Kit ELISA, AXSF Kit ELISA, growth arrest specific 6 Kit ELISA, growth arrest-specific 6 Kit ELISA, growth arrest specific 6 L homeolog Kit ELISA, growth arrest specific 6 S homeolog Kit ELISA, GAS6 Kit ELISA, gas6 Kit ELISA, gas6.L Kit ELISA, gas6.S Kit ELISA, Gas6 Kit ELISA

Sujet:

Protein Function: Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. .

Background: GAS6(Growth arrest-specific 6), also known as AXLLG or AXSF, is a human gene coding for the Gas6 protein. It is mapped to 13q34. GAS6 was originally found as a gene upregulated by growth arrested fibroblasts. This gene product is a gamma-carboxyglutamic acid(Gla)-containing protein which thought to be involved in the stimulation of cell proliferation, and may play a role in thrombosis. What's more, the product of the GAS6 gene, a ligand for the AXL, MER, and TYRO3 tyrosine kinase receptors, is a vitamin K-dependent protein, structurally related to anticoagulant protein S but lacking its anticoagulant activity. This protein also shows great importance in the cardiovascular system.

Synonyms: Growth arrest-specific protein 6,GAS-6,AXL receptor tyrosine kinase ligand,Gas6,

Full Gene Name: Growth arrest-specific protein 6

Cellular Localisation: Secreted.

ID gène: 14456

UniProt: Q61592

Pathways: Signalisation RTK, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response