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APCS Kit ELISA

APCS Reactivité: Rat AA 21-228 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1889378
  • Antigène Voir toutes APCS Kits ELISA
    APCS (Amyloid P Component, Serum (APCS))
    Épitope
    AA 21-228
    Reactivité
    • 9
    • 8
    • 7
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    1.56-100 ng/mL
    Seuil minimal de détection
    1.56 ng/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat SAP/PTX2
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: Q21-S228
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <50pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: Q21-S228
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the pentaxin family.

    Plaque
    Pre-coated
    Protocole
    rat SAP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SAP has been precoated onto 96-well plates. Standards(NSO, Q21-S228) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat SAP amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL rat SAP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat SAP standard solution and each sample is measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 5.68, Standard deviation: 0.33, CV(%): 5.8
    • Sample 2: n=16, Mean(ng/ml): 21, Standard deviation: 0.882, CV(%): 4.2
    • Sample 3: n=16, Mean(ng/ml): 42, Standard deviation: 2.1, CV(%): 5,
    • Sample 1: n=24, Mean(ng/ml): 5.2, Standard deviation: 0.39, CV(%): 7.5
    • Sample 2: n=24, Mean(ng/ml): 24, Standard deviation: 1.296, CV(%): 5.4
    • Sample 3: n=24, Mean(ng/ml): 51, Standard deviation: 3.06, CV(%): 6
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes APCS Kits ELISA
    APCS (Amyloid P Component, Serum (APCS))
    Autre désignation
    APCS (APCS Produits)
    Synonymes
    PTX2 Kit ELISA, SAP Kit ELISA, Sap Kit ELISA, APCS Kit ELISA, FP Kit ELISA, SAP(FP) Kit ELISA, apcs Kit ELISA, ptx2 Kit ELISA, sap Kit ELISA, amyloid P component, serum Kit ELISA, serum amyloid P-component Kit ELISA, amyloid P component, serum protein L homeolog Kit ELISA, APCS Kit ELISA, Apcs Kit ELISA, apcs.L Kit ELISA
    Sujet

    Background: Amyloid P component, serum(SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component(AP), a 25 kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.

    Synonyms: Serum amyloid P-component,SAP,Apcs,Ptx2, Sap,

    Full Gene Name: Serum amyloid P-component

    Cellular Localisation: Secreted.
    ID gène
    29339
    UniProt
    P23680
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