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CD13 Kit ELISA

ANPEP Reactivité: Humain AA 69-967 Colorimetric Sandwich ELISA 312-20.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1889381
  • Antigène Voir toutes CD13 (ANPEP) Kits ELISA
    CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))
    Épitope
    AA 69-967
    Reactivité
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    312-20.000 pg/mL
    Seuil minimal de détection
    312 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD13/Aminopeptidase N
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: K69-K967
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: K69-K967
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: Expressed in epithelial cells of the kidney, intestine, and respiratory tract, granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood- brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.

    Plaque
    Pre-coated
    Protocole
    human CD13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD13 has been precoated onto 96-well plates. Standards(NSO, K69-K967) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD13 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human CD13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD13 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 2.98, Standard deviation: 0.158, CV(%): 5.3
    • Sample 2: n=16, Mean(ng/ml): 6.54, Standard deviation: 0.360, CV(%): 5.5
    • Sample 3: n=16, Mean(ng/ml): 12.73, Standard deviation: 0.751, CV(%): 5.9,
    • Sample 1: n=24, Mean(ng/ml): 3.27, Standard deviation: 0.190, CV(%): 5.8
    • Sample 2: n=24, Mean(ng/ml): 6.83, Standard deviation: 0.430, CV(%): 6.3
    • Sample 3: n=24, Mean(ng/ml): 13.76, Standard deviation: 0.922, CV(%): 6.7
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Wang, Li, Sun, Ni, Huan, Huang, Li, Fan, Ren, Mao: "Triggering unfolded protein response by 2-Deoxy-D-glucose inhibits porcine epidemic diarrhea virus propagation." dans: Antiviral research, Vol. 106, pp. 33-41, (2014) (PubMed).

  • Antigène Voir toutes CD13 (ANPEP) Kits ELISA
    CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))
    Autre désignation
    ANPEP (ANPEP Produits)
    Synonymes
    APN Kit ELISA, CD13 Kit ELISA, GP150 Kit ELISA, LAP1 Kit ELISA, P150 Kit ELISA, PEPN Kit ELISA, A2AP Kit ELISA, AAP Kit ELISA, ALPHA-2-PI Kit ELISA, API Kit ELISA, PLI Kit ELISA, AAT1 Kit ELISA, ALT1 Kit ELISA, GPT1 Kit ELISA, Gpt1 Kit ELISA, Apn Kit ELISA, pepN Kit ELISA, SC8E4A.13 Kit ELISA, 1300007J06Rik Kit ELISA, 2310022B03Rik Kit ELISA, ALT Kit ELISA, Gpt-1 Kit ELISA, AP-M Kit ELISA, AP-N Kit ELISA, Cd13 Kit ELISA, Apm Kit ELISA, KZP Kit ELISA, Lap1 Kit ELISA, rAPN Kit ELISA, APDE Kit ELISA, p150 Kit ELISA, anpep Kit ELISA, fb64c05 Kit ELISA, wu:fb64c05 Kit ELISA, zgc:136771 Kit ELISA, alanyl aminopeptidase, membrane Kit ELISA, serpin family F member 2 Kit ELISA, glutamic--pyruvic transaminase Kit ELISA, aminopeptidase N Kit ELISA, aminopeptidase Kit ELISA, aminopeptidase n Kit ELISA, glutamic pyruvic transaminase, soluble Kit ELISA, alanyl (membrane) aminopeptidase Kit ELISA, membrane alanyl aminopeptidase Kit ELISA, alanyl (membrane) aminopeptidase b Kit ELISA, alanyl aminopeptidase, membrane L homeolog Kit ELISA, ANPEP Kit ELISA, SERPINF2 Kit ELISA, GPT Kit ELISA, Gpt Kit ELISA, Apn2 Kit ELISA, Apn1 Kit ELISA, SCO2643 Kit ELISA, pepN Kit ELISA, Shewana3_0897 Kit ELISA, Alvin_1024 Kit ELISA, Ndas_3413 Kit ELISA, Saut_1544 Kit ELISA, Fbal_1877 Kit ELISA, ML5_3424 Kit ELISA, Despr_1988 Kit ELISA, Mesop_5365 Kit ELISA, Anpep Kit ELISA, LOC101744250 Kit ELISA, anpepb Kit ELISA, anpep.L Kit ELISA
    Sujet

    Protein Function: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. .

    Background: Alanine aminopeptidase, also known as ANPEP or CD13. is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine, Aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases, and it is also thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Petrovic et al showed that CD13 was required for endothelial cell invasion in response to bradykinin. Inhibition of CD13 abrogated internalization of bradykinin receptor B2 and reduced endothelial cell motility.

    Synonyms: Aminopeptidase N,AP-N,hAPN,3.4.11.2,Alanyl aminopeptidase,Aminopeptidase M,AP-M,Microsomal aminopeptidase,Myeloid plasma membrane glycoprotein CD13,gp150,CD13,ANPEP,APN, CD13, PEPN,

    Full Gene Name: Aminopeptidase N

    Cellular Localisation: Cell membrane, Single-pass type II membrane protein. Cytoplasm, cytosol . A soluble form has also been detected.
    ID gène
    290
    UniProt
    P15144
    Pathways
    Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
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