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CCL9 Kit ELISA

Ccl9 Reactivité: Souris AA 22-122 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1889389
  • Antigène Voir toutes CCL9 (Ccl9) Kits ELISA
    CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))
    Épitope
    AA 22-122
    Reactivité
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    7.8-500 pg/mL
    Seuil minimal de détection
    7.8 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL9
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: E.coli
    Immunogen sequence: Q22-Q122
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <1pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: E.coli
    Immunogen sequence: Q22-Q122
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: Expressed mainly in the liver, lung, and the thymus, although some expression has been detected in a wide variety of tissues except brain.

    Plaque
    Pre-coated
    Protocole
    mouse CCL9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL9 has been precoated onto 96-well plates. Standards(E.coli, Q22-Q122) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL9 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse CCL9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse CCL9 standard solution and each sample is measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 34, Standard deviation: 1.19, CV(%): 3.5
    • Sample 2: n=16, Mean(pg/ml): 78, Standard deviation: 3.67, CV(%): 4.7
    • Sample 3: n=16, Mean(pg/ml): 294, Standard deviation: 14.99, CV(%): 5.1,
    • Sample 1: n=24, Mean(pg/ml): 35, Standard deviation: 1.68, CV(%): 4.8
    • Sample 2: n=24, Mean(pg/ml): 82, Standard deviation: 4.59, CV(%): 5.6
    • Sample 3: n=24, Mean(pg/ml): 302, Standard deviation: 19.03, CV(%): 6.3
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes CCL9 (Ccl9) Kits ELISA
    CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))
    Autre désignation
    Ccl9 (Ccl9 Produits)
    Synonymes
    CCF18 Kit ELISA, MRP-2 Kit ELISA, Scya10 Kit ELISA, Scya9 Kit ELISA, chemokine (C-C motif) ligand 9 Kit ELISA, Ccl9 Kit ELISA
    Sujet

    Protein Function: Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors.

    Background: Chemokine(C-C motif) ligand 9(CCL9), also called MIP-1 gamma and MRP-2, is a small cytokine belonging to the CC chemokine family. CCL9 which found around Peyer's patches is secreted by follicle-associated epithelium(FAE), and it can attract dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1. CCL9 is constitutively expressed in macrophages and myeloid cells. CCL9 can activate osteoclasts through its receptor CCR1(the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption.

    Synonyms: C-C motif chemokine 9,CCF18,Macrophage inflammatory protein 1-gamma,MIP-1-gamma,Macrophage inflammatory protein-related protein 2,MRP-2,Small-inducible cytokine A9,CCL9(29-101),CCL9(30-101),CCL9(31-101),Ccl9,Mrp2, Scya10, Scya9,

    Full Gene Name: C-C motif chemokine 9

    Cellular Localisation: Secreted.
    ID gène
    20308
    UniProt
    P51670
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