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CD320 Kit ELISA

CD320 Reactivité: Humain AA 36-229 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
N° du produit ABIN1889407
  • Antigène Voir toutes CD320 Kits ELISA
    CD320 (CD320 Molecule (CD320))
    Épitope
    AA 36-229
    Reactivité
    • 5
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156-10.000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD320/8D6A
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: S36-Y229
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: S36-Y229
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: Expressed abundantly on follicular dendritic cells (FDCs). .

    Plaque
    Pre-coated
    Protocole
    human CD320 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD320 has been precoated onto 96-well plates. Standards(NSO, S36-Y229) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD320 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD320 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CD320 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD320 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 1.1, Standard deviation: 0.042, CV(%): 3.8
    • Sample 2: n=16, Mean(ng/ml): 3.2, Standard deviation: 0.14, CV(%): 4.5
    • Sample 3: n=16, Mean(ng/ml): 6.3, Standard deviation: 0.31, CV(%): 4.9,
    • Sample 1: n=24, Mean(ng/ml): 1.3, Standard deviation: 0.08, CV(%): 6.2
    • Sample 2: n=24, Mean(ng/ml): 3.4, Standard deviation: 0.194, CV(%): 5.7
    • Sample 3: n=24, Mean(ng/ml): 5.9, Standard deviation: 0.313, CV(%): 5.3
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes CD320 Kits ELISA
    CD320 (CD320 Molecule (CD320))
    Autre désignation
    CD320 (CD320 Produits)
    Synonymes
    8D6 Kit ELISA, 8D6A Kit ELISA, TCBLR Kit ELISA, 425O18-1 Kit ELISA, D17Ertd716e Kit ELISA, NG29 Kit ELISA, VLDL Kit ELISA, RGD1305860 Kit ELISA, TCblR Kit ELISA, CD320 molecule Kit ELISA, CD320 antigen Kit ELISA, CD320 Kit ELISA, Cd320 Kit ELISA
    Sujet

    Protein Function: Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC). CD320 augments the proliferation of PC precursors generated by IL-10. Receptor for the cellular uptake of transcobalamin bound cobalamin. .

    Background: CD320(cluster of differentiation 320), also known as 8D6A or TCBLR, is a human gene. It is mapped to chromosome 19p13.2. This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin(vitamin B12), and the expression of CD320 enhanced B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. It has been found that antagonists of the CD320 signaling pathway may counter the growth of follicular lymphomas or other tumors that metastasize to lymphoid follicles. CD320 is also a specific receptor for uptake of TCN2-bound cobalamin.

    Synonyms: CD320 antigen,8D6 antigen,FDC-signaling molecule 8D6,FDC-SM-8D6,Transcobalamin receptor,TCblR,CD320,CD320,8D6A,UNQ198/PRO224,

    Full Gene Name: CD320 antigen

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    ID gène
    51293
    UniProt
    Q9NPF0
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