IL18R1 Kit ELISA

N° du produit ABIN1889411

Détail du produit IL18R1 Kit ELISA

Antigène: IL18R1 (Interleukin 18 Receptor 1 (IL18R1))

Épitope: AA 22-329

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-18R1

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: C22-R329

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: C22-R329

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the interleukin-1 receptor family.

Tissue Specificity: Expressed in lung, leukocytes, spleen, liver, thymus, prostate, small intestine, colon, placenta, and heart, and is absent from brain, skeletal muscle, pancreas, and kidney. High level of expression in Hodgkin disease cell lines.

Plaque: Pre-coated

Protocole: human IL-18R1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18R1 has been precoated onto 96-well plates. Standards(NSO, C22-R329) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-18R1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-18R1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL-18R1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-18R1 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 250, Standard deviation: 13.2, CV(%): 5.3
• Sample 2: n=16, Mean(pg/ml): 625, Standard deviation: 38.7, CV(%): 6.2
• Sample 3: n=16, Mean(pg/ml): 1255, Standard deviation: 70.2, CV(%): 5.6,
• Sample 1: n=24, Mean(pg/ml): 253, Standard deviation: 20.7, CV(%): 8.2
• Sample 2: n=24, Mean(pg/ml): 612, Standard deviation: 45.9, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 1078, Standard deviation: 76.5, CV(%): 7.1

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: IL18R1 (Interleukin 18 Receptor 1 (IL18R1))

Autre désignation: IL18R1 (IL18R1 Produits)

Synonymes: CD218a Kit ELISA, CDw218a Kit ELISA, IL-1Rrp Kit ELISA, IL18RA Kit ELISA, IL1RRP Kit ELISA, IL-1R9 Kit ELISA, IL1R9 Kit ELISA, IL1RAPL-2 Kit ELISA, TIGIRR-1 Kit ELISA, Il18ralpha Kit ELISA, Il1rrp Kit ELISA, IL-18Ra Kit ELISA, IL18R1 Kit ELISA, interleukin 18 receptor 1 Kit ELISA, interleukin 1 receptor accessory protein like 2 Kit ELISA, IL18R1 Kit ELISA, IL1RAPL2 Kit ELISA, Il18r1 Kit ELISA

Sujet:

Protein Function: Receptor for interleukin 18 (IL-18). Binding to the agonist leads to the activation of NF-kappa-B.

Background: The interleukin-18 receptor 1(IL-18R1), also known as CDw218a(cluster of differentiation w218a) or IL18RA, is an interleukin receptor of the immunoglobulin superfamily. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2(IL-1Rrp2), and IL1RL1(T1/ST2), form a gene cluster on chromosome 2q. This receptor specifically binds interleukin 18(IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells.

Synonyms: Interleukin-18 receptor 1,IL-18R-1,IL-18R1,CD218 antigen-like family member A,CDw218a,IL1 receptor-related protein,IL-1Rrp,IL1R-rp,CD218a,IL18R1,IL1RRP,

Full Gene Name: Interleukin-18 receptor 1

Cellular Localisation: Membrane, Single-pass type I membrane protein.

ID gène: 8809

UniProt: Q13478