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LBP Kit ELISA

LBP Reactivité: Souris AA 25-481 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1889416
  • Antigène Voir toutes LBP Kits ELISA
    LBP (Lipopolysaccharide Binding Protein (LBP))
    Épitope
    AA 25-481
    Reactivité
    • 10
    • 9
    • 7
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    1.56-100 ng/mL
    Seuil minimal de détection
    1.56 ng/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse LBP
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: G25-V481
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <50pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: G25-V481
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plaque
    Pre-coated
    Protocole
    mouse LBP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for LBP has been precoated onto 96-well plates. Standards(NSO, G25-V481) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LBP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse LBP amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL mouse LBP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse LBP standard solution and each sample is measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 5.61, Standard deviation: 0.32, CV(%): 5.7
    • Sample 2: n=16, Mean(ng/ml): 33.8, Standard deviation: 1.55, CV(%): 4.6
    • Sample 3: n=16, Mean(ng/ml): 68.2, Standard deviation: 3.41, CV(%): 5,
    • Sample 1: n=24, Mean(ng/ml): 5.32, Standard deviation: 0.46, CV(%): 8.6
    • Sample 2: n=24, Mean(ng/ml): 36.5, Standard deviation: 1.93, CV(%): 5.3
    • Sample 3: n=24, Mean(ng/ml): 74.9, Standard deviation: 4.81, CV(%): 6.4
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Wang, Shen, Zhang, Wang, Xu, Chen, Chen, Yang, He, Wang, Su, Cheng, Zhao, Wang: "Reduction Impairs the Antibacterial Activity but Benefits the LPS Neutralization Ability of Human Enteric Defensin 5." dans: Scientific reports, Vol. 6, pp. 22875, (2017) (PubMed).

  • Antigène Voir toutes LBP Kits ELISA
    LBP (Lipopolysaccharide Binding Protein (LBP))
    Autre désignation
    LBP (LBP Produits)
    Synonymes
    BPIFD2 Kit ELISA, Bpifd2 Kit ELISA, Ly88 Kit ELISA, LPSBP Kit ELISA, LBP Kit ELISA, lipopolysaccharide binding protein Kit ELISA, lipopolysaccharide-binding protein Kit ELISA, LBP Kit ELISA, Lbp Kit ELISA, LOC100472839 Kit ELISA
    Sujet

    Protein Function: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide (By similarity). .

    Background: Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide(or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.

    Synonyms: Lipopolysaccharide-binding protein,LBP,Lbp,

    Full Gene Name: Lipopolysaccharide-binding protein

    Cellular Localisation: Secreted.
    ID gène
    16803
    UniProt
    Q61805
    Pathways
    Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process
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