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DLL1 Kit ELISA

DLL1 Reactivité: Humain AA 22-540 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Serum, Urine
N° du produit ABIN1889418
  • Antigène Voir toutes DLL1 Kits ELISA
    DLL1 (delta-Like 1 (DLL1))
    Épitope
    AA 22-540
    Reactivité
    • 6
    • 3
    • 3
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    78-5000 pg/mL
    Seuil minimal de détection
    78 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human DLL1
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine, Milk
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: S22-G540
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: S22-G540
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Contains 1 DSL domain.

    Tissue Specificity: Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.

    Plaque
    Pre-coated
    Protocole
    human DLL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DLL1 has been precoated onto 96-well plates. Standards(NSO, S22-G540) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DLL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DLL1 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human DLL1 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), urine or human milk to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human DLL1 standard solution and each sample is measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 713, Standard deviation: 24.9, CV(%): 3.5
    • Sample 2: n=16, Mean(pg/ml): 1579, Standard deviation: 64.74, CV(%): 4.1
    • Sample 3: n=16, Mean(pg/ml): 3618, Standard deviation: 137.5, CV(%): 3.8,
    • Sample 1: n=24, Mean(pg/ml): 674, Standard deviation: 49.8, CV(%): 7.4
    • Sample 2: n=24, Mean(pg/ml): 1486, Standard deviation: 101, CV(%): 6.8
    • Sample 3: n=24, Mean(pg/ml): 3542, Standard deviation: 269.2, CV(%): 7.6
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes DLL1 Kits ELISA
    DLL1 (delta-Like 1 (DLL1))
    Autre désignation
    DLL1 (DLL1 Produits)
    Synonymes
    X-delta-1 Kit ELISA, XDelta1 Kit ELISA, Xdelta-1 Kit ELISA, delta Kit ELISA, delta-1 Kit ELISA, delta1 Kit ELISA, x-delta Kit ELISA, DELTA1 Kit ELISA, DL1 Kit ELISA, Delta Kit ELISA, DLK Kit ELISA, DLK-1 Kit ELISA, Delta1 Kit ELISA, FA1 Kit ELISA, PREF1 Kit ELISA, Pref-1 Kit ELISA, ZOG Kit ELISA, pG2 Kit ELISA, DLL1 Kit ELISA, AW742678 Kit ELISA, DlkI Kit ELISA, Ly107 Kit ELISA, Peg9 Kit ELISA, SCP1 Kit ELISA, pref-1 Kit ELISA, delta-like 1 Kit ELISA, delta like canonical Notch ligand 1 Kit ELISA, delta like non-canonical Notch ligand 1 Kit ELISA, delta-like 1 L homeolog Kit ELISA, delta-like 1 (Drosophila) Kit ELISA, delta-like 1 homolog (Drosophila) Kit ELISA, dll1 Kit ELISA, DLL1 Kit ELISA, DLK1 Kit ELISA, Dll1 Kit ELISA, dll1.L Kit ELISA, Dlk1 Kit ELISA
    Sujet

    Protein Function: Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. .

    Background: DLL1(DELTA-LIKE 1), also known as DL1 or DELTA1, is a protein that in humans is encoded by the DLL1 gene. DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis, and it is also important in cell-to-cell communication. This gene is mapped to chromosome 6q27, near a locus for type I diabetes. Functional analysis suggested that a soluble fusion protein containing the DSL domain of DLL1 and its adjacent 50 N-terminal amino acids increased the viability of hemopoietic cells but inhibited cell death. It has been found that DLL1 blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

    Synonyms: Delta-like protein 1,Drosophila Delta homolog 1,Delta1,H-Delta-1,DLL1,UNQ146/PRO172,

    Full Gene Name: Delta-like protein 1

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    ID gène
    28514
    UniProt
    O00548
    Pathways
    Signalisation Notch, Stem Cell Maintenance
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