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LGALS7 Kit ELISA

LGALS7 Reactivité: Souris AA 2-136 Colorimetric Sandwich ELISA 93.8-6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1889421
  • Antigène Voir toutes LGALS7 Kits ELISA
    LGALS7 (Galectin 7 (LGALS7))
    Épitope
    AA 2-136
    Reactivité
    • 7
    • 7
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    93.8-6000 pg/mL
    Seuil minimal de détection
    93.8 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Galectin-7
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: E.coli
    Immunogen sequence: S2-F136
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: E.coli
    Immunogen sequence: S2-F136
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plaque
    Pre-coated
    Protocole
    mouse Galectin-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Galectin-7 has been precoated onto 96-well plates. Standards(E.coli, S2-F136) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Galectin-7 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.8pg/mL mouse Galectin-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Galectin-7 standard solution and each sample is measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 356, Standard deviation: 16.4, CV(%): 4.6
    • Sample 2: n=16, Mean(pg/ml): 2745, Standard deviation: 156.5, CV(%): 5.7
    • Sample 3: n=16, Mean(pg/ml): 5032, Standard deviation: 191.2, CV(%): 3.8,
    • Sample 1: n=24, Mean(pg/ml): 468, Standard deviation: 33.7, CV(%): 7.2
    • Sample 2: n=24, Mean(pg/ml): 2012, Standard deviation: 1710.2, CV(%): 8.5
    • Sample 3: n=24, Mean(pg/ml): 4584, Standard deviation: 289, CV(%): 6.3
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes LGALS7 Kits ELISA
    LGALS7 (Galectin 7 (LGALS7))
    Autre désignation
    LGALS7 (LGALS7 Produits)
    Synonymes
    GAL7 Kit ELISA, LGALS7A Kit ELISA, Galectin-7 Kit ELISA, Pig1 Kit ELISA, LGALS7 Kit ELISA, galectin-7 Kit ELISA, galectin 7 Kit ELISA, lectin, galactose binding, soluble 7 Kit ELISA, galectin-7 Kit ELISA, LGALS7 Kit ELISA, Lgals7 Kit ELISA, LOC101112249 Kit ELISA
    Sujet

    Protein Function: Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release (By similarity). .

    Background: Galectin-7, also known as GAL7, is a protein that in humans is encoded by the LGALS7 gene. This gene is mapped to 19q13.2. Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It has been found that expression of Galectin-7 was markedly upregulated in corneal epithelium after injury and that exogenous galectin-7 stimulated reepithelialization of corneal wounds.

    Synonyms: Galectin-7,Gal-7,Lgals7,

    Full Gene Name: Galectin-7

    Cellular Localisation: Cytoplasm . Nucleus . Secreted . May be secreted by a non-classical secretory pathway..
    ID gène
    16858
    UniProt
    O54974
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