Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

GNLY Kit ELISA

GNLY Reactivité: Humain AA 1-145 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1889422
  • Antigène Voir toutes GNLY Kits ELISA
    GNLY (Granulysin (GNLY))
    Épitope
    AA 1-145
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2-2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Granulysin
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: M1-L145
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: M1-L145
    Featured
    Discover our best selling GNLY Kit ELISA
    Top Product
    Discover our top product GNLY Kit ELISA
  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: Expressed in natural killer and T-cells.

    Plaque
    Pre-coated
    Protocole
    human Granulysin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Granulysin has been precoated onto 96-well plates. Standards(NSO, M1-L145) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Granulysin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Granulysin amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Granulysin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Granulysin standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 355, Standard deviation: 20.59, CV(%): 5.8
    • Sample 2: n=16, Mean(pg/ml): 862, Standard deviation: 38.79, CV(%): 4.5
    • Sample 3: n=16, Mean(pg/ml): 1513, Standard deviation: 65, CV(%): 4.3,
    • Sample 1: n=24, Mean(pg/ml): 324, Standard deviation: 26.5, CV(%): 8.2
    • Sample 2: n=24, Mean(pg/ml): 765, Standard deviation: 55.85, CV(%): 7.3
    • Sample 3: n=24, Mean(pg/ml): 1322, Standard deviation: 89.8, CV(%): 6.8
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes GNLY Kits ELISA
    GNLY (Granulysin (GNLY))
    Autre désignation
    GNLY (GNLY Produits)
    Synonymes
    519 Kit ELISA, D2S69E Kit ELISA, LAG-2 Kit ELISA, LAG2 Kit ELISA, NKG5 Kit ELISA, TLA519 Kit ELISA, granulysin Kit ELISA, GNLY Kit ELISA
    Sujet

    Protein Function: Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.

    Background: Granulysin is a substance released by cytotoxic T cells(CD8) when they are attached to infected body cells. The product of this gene is a member of the saposin-like protein(SAPLIP) family. It is mapped to 2p11.2. Granulysin functions to create holes in the target cell membrane and destroy it. It is able to induce apoptosis in target cells and also has antimicrobial action. This gene is expressed in cytolytic granules with perforin, a pore forming protein, and granzymes that are also involved in cytolysis. In addition to it, Granulysin is broadly antimicrobial, killing microbes that cause, for example, tuberculosis and malaria, and can destroy some tumors. A series of peptides generated from the amino acid sequence of Granulysin are potential antibiotics. It has been found that secretory Granulysin is a key molecule responsible for the disseminated keratinocyte death in SJS/TEN.

    Synonyms: Granulysin,Lymphokine LAG-2,Protein NKG5,T-cell activation protein 519,GNLY,LAG2, NKG5, TLA519,

    Full Gene Name: Granulysin

    Cellular Localisation: Secreted. Located in the cytotoxic granules of T-cells, which are released upon antigen stimulation.
    ID gène
    10578
    UniProt
    P22749
Vous êtes ici:
Support technique