Angiopoietin 1 Kit ELISA

N° du produit ABIN1889436

Détail du produit Angiopoietin 1 Kit ELISA

Antigène: Angiopoietin 1 (ANGPT1)

Épitope: AA 16-497

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10.000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Angiopoietin-1

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: H16-F497

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: H16-F497

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse Angiopoietin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Angiopoietin-1 has been precoated onto 96-well plates. Standards(NSO, H16-F497) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiopoietin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Angiopoietin-1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Angiopoietin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Angiopoietin-1 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 1.28, Standard deviation: 0.055, CV(%): 4.3
• Sample 2: n=16, Mean(ng/ml): 3.54, Standard deviation: 0.131, CV(%): 3.7
• Sample 3: n=16, Mean(ng/ml): 6.85, Standard deviation: 0.301, CV(%): 4.4,
• Sample 1: n=24, Mean(ng/ml): 1.59, Standard deviation: 0.086, CV(%): 5.4
• Sample 2: n=24, Mean(ng/ml): 3.83, Standard deviation: 0.181, CV(%): 4.7
• Sample 3: n=24, Mean(ng/ml): 6.97, Standard deviation: 0.369, CV(%): 5.3

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour Angiopoietin 1 Kit ELISA (ABIN1889436)

Schlosser, Taha, Deng, McIntyre, Mei, Stewart: "High circulating angiopoietin-2 levels exacerbate pulmonary inflammation but not vascular leak or mortality in endotoxin-induced lung injury in mice." dans: Thorax, Vol. 73, Issue 3, pp. 248-261, (2018) (PubMed).

Burnett, Gomez, De Leon, Ariaans, Progias, Kammerer, Velasco, Marron, Hellewell, Ridger: "Angiopoietin-1 enhances neutrophil chemotaxis in vitro and migration in vivo through interaction with CD18 and release of CCL4." dans: Scientific reports, Vol. 7, Issue 1, pp. 2332, (2017) (PubMed).

Détail du antigène

Antigène: Angiopoietin 1 (ANGPT1)

Autre désignation: ANGPT1 (ANGPT1 Produits)

Synonymes: AGP1 Kit ELISA, AGPT Kit ELISA, ANG1 Kit ELISA, 1110046O21Rik Kit ELISA, Ang-1 Kit ELISA, Ang1 Kit ELISA, Agpt Kit ELISA, Agpt1 Kit ELISA, ang1 Kit ELISA, wu:fc76g06 Kit ELISA, ANG-1 Kit ELISA, angpt1 Kit ELISA, MGC80788 Kit ELISA, angiopoietin 1 Kit ELISA, angiopoietin 1 S homeolog Kit ELISA, ANGPT1 Kit ELISA, Angpt1 Kit ELISA, angpt1 Kit ELISA, angpt1.S Kit ELISA

Sujet:

Protein Function: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme (By similarity). .

Background: Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

Synonyms: Angiopoietin-1,ANG-1,Angpt1,Agpt,

Full Gene Name: Angiopoietin-1

Cellular Localisation: Secreted.

ID gène: 11600

UniProt: O08538

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process