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CD48 Kit ELISA

CD48 Reactivité: Souris AA 23-217 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1889451
  • Antigène Voir toutes CD48 Kits ELISA
    CD48
    Épitope
    AA 23-217
    Reactivité
    • 5
    • 4
    • 2
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    62.5-4000 pg/mL
    Seuil minimal de détection
    62.5 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD48
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: F23-S217
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: F23-S217
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

    Plaque
    Pre-coated
    Protocole
    mouse CD48 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD48 has been precoated onto 96-well plates. Standards(NSO, F23-S217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD48 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD48 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse CD48 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD48 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 382, Standard deviation: 13.37, CV(%): 3.5
    • Sample 2: n=16, Mean(pg/ml): 1554, Standard deviation: 65.27, CV(%): 4.2
    • Sample 3: n=16, Mean(pg/ml): 2790, Standard deviation: 131.13, CV(%): 4.7,
    • Sample 1: n=24, Mean(pg/ml): 426, Standard deviation: 19.17, CV(%): 4.5
    • Sample 2: n=24, Mean(pg/ml): 1713, Standard deviation: 90.79, CV(%): 5.3
    • Sample 3: n=24, Mean(pg/ml): 3225, Standard deviation: 187.05, CV(%): 5.8
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes CD48 Kits ELISA
    CD48
    Autre désignation
    CD48 (CD48 Produits)
    Synonymes
    AI449234 Kit ELISA, AW610730 Kit ELISA, BCM1 Kit ELISA, BLAST Kit ELISA, BLAST-1 Kit ELISA, BLAST1 Kit ELISA, Bcm-1 Kit ELISA, MEM-102 Kit ELISA, SLAMF2 Kit ELISA, Sgp-60 Kit ELISA, hCD48 Kit ELISA, mCD48 Kit ELISA, CD48 antigen Kit ELISA, Cd48 molecule Kit ELISA, CD48 molecule Kit ELISA, Cd48 Kit ELISA, CD48 Kit ELISA
    Sujet

    Protein Function: Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.

    Background: CD48 antigen(Cluster of Differentiation 48), also known as BLAST-1 or SLAMF2, is a protein that in humans is encoded by the CD48 gene. CD48 is a member of the CD2 subfamily of the immunoglobulin superfamily(IgSF) which includes SLAM(signaling lymphocyte activation molecules) proteins, such as CD84, CD150, CD229 and CD244. It is mapped to 1q23.3. CD48 is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor.

    Synonyms: CD48 antigen,BCM1 surface antigen,BLAST-1,HM48-1,MRC OX-45 surface antigen,SLAM family member 2,SLAMF2,Signaling lymphocytic activation molecule 2,sgp-60,CD48,Cd48,Bcm-1,

    Full Gene Name: CD48 antigen

    Cellular Localisation: Cell membrane, Lipid-anchor, GPI-anchor.
    ID gène
    12506
    UniProt
    P18181
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