CD21 Kit ELISA

N° du produit ABIN1889452

Détail du produit CD21 Kit ELISA

Antigène: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Épitope: AA 21-71

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78-50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD21/CR2

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: CHO
Immunogen sequence: Ile21-71

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <20pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: CHO
Immunogen sequence: Ile21-71

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the receptors of complement activation (RCA) family.

Tissue Specificity: Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.

Plaque: Pre-coated

Protocole: human CD21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD21 has been precoated onto 96-well plates. Standards(CHO, Ile21-71) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD21 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human CD21 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD21 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 9.7, Standard deviation: 0.44, CV(%): 4.5
• Sample 2: n=16, Mean(ng/ml): 18.4, Standard deviation: 0.77, CV(%): 4.2
• Sample 3: n=16, Mean(ng/ml): 34.5, Standard deviation: 1.24, CV(%): 3.6,
• Sample 1: n=24, Mean(ng/ml): 12.2, Standard deviation: 0.77, CV(%): 6.3
• Sample 2: n=24, Mean(ng/ml): 21.6, Standard deviation: 1.23, CV(%): 5.7
• Sample 3: n=24, Mean(ng/ml): 38.8, Standard deviation: 1.86, CV(%): 4.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Autre désignation: CR2 (CR2 Produits)

Synonymes: C3DR Kit ELISA, CD21 Kit ELISA, CR Kit ELISA, CVID7 Kit ELISA, SLEB9 Kit ELISA, Cd21 Kit ELISA, cd21 Kit ELISA, CR1 Kit ELISA, CR1-L Kit ELISA, CR2 Kit ELISA, complement C3d receptor 2 Kit ELISA, complement C3b/C4b receptor 1 (Knops blood group) Kit ELISA, CR2 Kit ELISA, Cr2 Kit ELISA, CR1 Kit ELISA

Sujet:

Protein Function: Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation. .

Background: Complement receptor type 2(CR2), also known as C3DR or CD21, is a protein that in humans is encoded by the CR2 gene. It is mapped to 1q32.2. CR2 is involved in the complement system. It binds to iC3b(inactive derivative of C3b), C3dg, or C3d. CR2 is the membrane protein on B lymphocytes to which the Epstein-Barr virus (EBV) binds during infection of these cells. CR2, together with CD19, CD81, and CD225, forms the B-cell coreceptor complex, which lowers the activation threshold of the B-cell antigen receptor. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9).

Synonyms: Complement receptor type 2,Cr2,Complement C3d receptor,Epstein-Barr virus receptor,EBV receptor,CD21,CR2,C3DR,

Full Gene Name: Complement receptor type 2

Cellular Localisation: Membrane, Single-pass type I membrane protein.

ID gène: 1380

UniProt: P20023

Pathways: Système du Complément