EGFR Kit ELISA

N° du produit ABIN1981823

Détail du produit EGFR Kit ELISA

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Épitope: pTyr1086

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Fonction: Cell-Based Human/Mouse/Rat EGFR (Tyr845) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.

Marque: CellBIND®,RayBio®

Type d'échantillon: Cell Culture Cells

Analytical Method: Semi-Quantitative

Specificité: The antibodies provided in this kit recognizes human, mouse and rat EGFR phosphorylated at site Tyrosine-845 and total EGFR for comparison.

Attributs du produit:

• Site and signal pathway-specific
• In vitro detection of adherent cell culture
• No sample lysis needed
• Compatible with a standard ELISA plate reader
• Faster results than with ELISA
• Adaptable for high-throughput screening and drug discovery

Ingrédients:

• uncoated 96-well Microplate
• Wash Buffer A
• Wash Buffer B
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Uncoated

Protocole:

1. Seed 10,000-30,000 cells into each well and incubate overnight.
2. Apply various treatment, inhibitors or activators according to manufacture's instructions.
3. Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
4. Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
5. Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
6. Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
7. Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Préparation des réactifs:

NOTE: Thaw all reagents to room temperature immediately before use. If wash buffers contain visible crystals, warm to room temperature and mix gently until dissolved.
NOTE: Briefly centrifuge (~1,000g) ITEMS G, H, and I before opening to ensure maximum recovery.
For more information look at the picture.

Procédure de l'essai:

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1-2 cycles/sec).
1. Design your experiment. For example, see in Figure 2 below.
OPTIONAL: If seeding HUVECs, HMEC-1 or other loosely attached cells, coat the Uncoated 96-Well Microplate (ITEM A) by adding 100 µL poly-L-Lysine (Recommended Sigma Aldrich) into each well and then follow manufacturer's instructions. A pre-coated CellBIND® microplate or other poly-lysine treated tissue culture plate may be used in place of Item A.
2. Seed 100 µL of 30,000 cells into each well of the Uncoated 96-Well Microplate (ITEM A) provided and incubate overnight at 37 °C with 5 % CO2.
NOTE: The optimal cell number used will vary on the cell line and the relative amount of protein phosphorylation. More or less cells may be used but this must be determined empirically.
NOTE: The cells can be starved ~4-24 hours (depending on cell line) prior to treatment with inhibitors or activators.
3. Apply various treatments, inhibitors (such as siRNA or chemicals) or activators according to manufacturer's instructions and incubate for the desired time points.
NOTE: It is recommended to dissolve inhibitors or activators into serum-free cell culture medium before treating the cells (unless otherwise stated in the manufacturer's instructions.)
4. Discard the cell culture medium by flipping the microplate upside down and gently tapping the bottom of the microplate over a sink.
5. Wash by pipetting 200 µL of the prepared 1X Wash Buffer A (ITEM B) into each well. Discard the wash buffer (same as step 4) and wash 2 more times for a total of 3 washes using fresh wash buffer each time. After the final wash, gently blot the microplate onto a paper towel to remove any excess/remaining buffer.
NOTE: To avoid cell loss, do not pipette directly onto the cells. Instead, gently dispense the liquid down the wall of cell culture wells. Also avoid the use of vacuum suction or too forcefully tapping the microplate when discarding any solution.
6. Add 100 µL of Fixing Solution (ITEM D) into each well and incubate for 20 minutes at room temperature.
NOTE: The fixing solution is used to permeabilize the cells.
7. Repeat wash step 5.
8. Add 200 µL of the prepared 1X Quenching Buffer (ITEM E) into each well and incubate 20 minutes at room temperature.
NOTE: The quenching buffer is used to minimize the background response.
9. Wash 4 times with 1X Wash Buffer A.
10. Add 200 µL of the prepared 1X Blocking Buffer (ITEM F) into each well and incubate for 1 hour at 37 °C.
11. Wash 3 times with the prepared 1X Wash Buffer B (ITEM C).
NOTE: If needed, the microplate may be stored at -80 °C for several days after this wash.
12. Add 50 µL of the prepared 1X primary antibody (ITEM G or H) into each corresponding well and incubate for 2 hours at room temperature.
13. Wash 4 times with 1X Wash Buffer B.
14. Add 50 µL of 1X HRP Conjugated secondary antibody (ITEM I) into each well and incubate for 1 hour at room temperature.
15. Wash 4 times with 1X Wash Buffer B.
16. Add 100 µL of the TMB Substrate (ITEM J) into each well and incubate for 30 minutes at room temperature in the dark.
17. Add 50 µL of the Stop Solution (ITEM K) into each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Références pour EGFR Kit ELISA (ABIN1981823)

Biscardi, Maa, Tice, Cox, Leu, Parsons: "c-Src-mediated phosphorylation of the epidermal growth factor receptor on Tyr845 and Tyr1101 is associated with modulation of receptor function." dans: The Journal of biological chemistry, Vol. 274, Issue 12, pp. 8335-43, (1999) (PubMed).

Hackel, Zwick, Prenzel, Ullrich: "Epidermal growth factor receptors: critical mediators of multiple receptor pathways." dans: Current opinion in cell biology, Vol. 11, Issue 2, pp. 184-9, (1999) (PubMed).

Zwick, Hackel, Prenzel, Ullrich: "The EGF receptor as central transducer of heterologous signalling systems." dans: Trends in pharmacological sciences, Vol. 20, Issue 10, pp. 408-12, (1999) (PubMed).

Hubbard, Wei, Ellis, Hendrickson: "Crystal structure of the tyrosine kinase domain of the human insulin receptor." dans: Nature, Vol. 372, Issue 6508, pp. 746-54, (1995) (PubMed).

Détail du antigène

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation: EGFR (EGFR Produits)

Synonymes: C-erb Kit ELISA, CG10079 Kit ELISA, D-EGFR Kit ELISA, D-Egf Kit ELISA, DEGFR Kit ELISA, DER Kit ELISA, DER flb Kit ELISA, DER/EGFR Kit ELISA, DER/faint little ball Kit ELISA, DER/top Kit ELISA, DER/torpedo Kit ELISA, DER1 Kit ELISA, DEgfr Kit ELISA, Degfr Kit ELISA, Der Kit ELISA, DmHD-33 Kit ELISA, Dmel\\CG10079 Kit ELISA, EFG-R Kit ELISA, EGF-R Kit ELISA, EGFR Kit ELISA, EGFr Kit ELISA, EGfr Kit ELISA, EK2-6 Kit ELISA, Egf Kit ELISA, Egf-r Kit ELISA, EgfR Kit ELISA, El Kit ELISA, Elp Kit ELISA, Elp-1 Kit ELISA, Elp-B1 Kit ELISA, Elp-B1RB1 Kit ELISA, HD-33 Kit ELISA, TOP Kit ELISA, Torpedo/DER Kit ELISA, Torpedo/Egfr Kit ELISA, c-erbB Kit ELISA, d-egf-r Kit ELISA, dEGFR Kit ELISA, dEGFR1 Kit ELISA, dEgfr Kit ELISA, der Kit ELISA, egfr Kit ELISA, flb Kit ELISA, l(2)05351 Kit ELISA, l(2)09261 Kit ELISA, l(2)57DEFa Kit ELISA, l(2)57EFa Kit ELISA, l(2)57Ea Kit ELISA, mor1 Kit ELISA, top Kit ELISA, top/DER Kit ELISA, top/flb Kit ELISA, torpedo/Egfr Kit ELISA, torpedo/egfr Kit ELISA, EGFR12 Kit ELISA, EGFR15 Kit ELISA, egfr1 Kit ELISA, Erbb2 Kit ELISA, ERBB Kit ELISA, ERBB1 Kit ELISA, HER1 Kit ELISA, PIG61 Kit ELISA, mENA Kit ELISA, ErbB-1 Kit ELISA, Errp Kit ELISA, 9030024J15Rik Kit ELISA, AI552599 Kit ELISA, Erbb Kit ELISA, Errb1 Kit ELISA, Wa5 Kit ELISA, wa-2 Kit ELISA, wa2 Kit ELISA, epidermal growth factor receptor Kit ELISA, Epidermal growth factor receptor Kit ELISA, epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) Kit ELISA, EGFR Kit ELISA, Egfr Kit ELISA, egfra Kit ELISA, egfr1 Kit ELISA, LOC5564544 Kit ELISA

ID gène: 1956

UniProt: P00533

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins