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Anti-Nuclear Antibody (ANA) Kit ELISA

ANA Reactivité: Humain Colorimetric Plasma, Serum
N° du produit ABIN2648251
  • Antigène Voir toutes Anti-Nuclear Antibody (ANA) Kits ELISA
    Anti-Nuclear Antibody (ANA)
    Reactivité
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    • 1
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    • 1
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    Humain
    Méthode de détection
    Colorimetric
    Application
    ELISA
    Fonction
    Enzyme immunoassay for the determination of IgG antibodies to nuclear and cytoplasmic antigensin human serum and plasma
    Marque
    ANAscreen
    Type d'échantillon
    Serum, Plasma
    Analytical Method
    Semi-Quantitative
    Specificité
    Frequency distribution of ANA in ANAscreen: 97unselected human sera from healthy donors weretested. All sera were found negative. Thiscorresponds to a diagnostic specificity of 100%.
    Attributs du produit
    ANAscreenallows the detection of total autoantibodiesto nuclear and cytoplasmic antigens in one sample as asummary parameter in the diagnosis of systemicautoimmunedisorders.Antigeniccombinationofcomplete HeLa nuclei with recombinant proteins andpurified native antigens guarantees a maximum ofsensitivy and specificity for the ANA detection.
    Ingrédients
    Microtiter plate
    Concentrated wash buffer
    Sample diluent
    Conjugate
    Substrate
    Stop solution
    Positive Control
    Cut-off Control
    Negative Control
    Matériel non inclus
    micropipettes
    multi-channel pipette or multi-pipettetrough for multi-channel pipette
    8-channel wash comb with vacuum pump and wastebottle or microplate washer
    distilled or de-ionized water
    glassware
    Top Product
    Discover our top product ANA Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    The ANAscreen ELISA Assay Kit is an enzymeimmunoassay for the semi-quantitative determination of IgG antibodies to nuclear and cytoplasmic antigens. Antibodies of the controls and diluted patient samples react with nuclear and cytoplasmic antigens immobilized on the solid phase of microtiter plates. Use of complete HeLa nuclei enriched with recombinant and native antigens guarantees the specific binding of autoimmune antibodies of the specimen under investigation. Following an incubation period of 60 min at room temperature (RT,18...25°C),unbound sample components are removed by a wash step. The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish peroxidase (HRP). Within the incubation period of 30 min at RT, excessive conjugate is separated from the solid-phase immunecomplexes by the following wash step. HRP converts the colorless substrate solution of 3,3’,5,5’-tetramethylbenzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution into the wells after 15 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. Patient ratios are calculated by dividing the respective OD of the specimen with the calculated cut-off OD.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Stockage commentaire
    Upon receipt, all components of the ANAscreen have tobe kept at 2 - 8 °C, preferably in the original kit box.
  • Antigène Voir toutes Anti-Nuclear Antibody (ANA) Kits ELISA
    Anti-Nuclear Antibody (ANA)
    Autre désignation
    ANA (Antinuclear Antibody) (ANA Produits)
    Synonymes
    ANA Kit ELISA, TOB5 Kit ELISA, TOB55 Kit ELISA, TOFA Kit ELISA, BTG anti-proliferation factor 3 Kit ELISA, BTG3 Kit ELISA
    Classe de substances
    Antibody, Antibody
    Sujet
    Systemic autoimmune diseases such as systemic lupuserythematosus,scleroderma,rheumatoidarthritis,Sjögren’s syndrome, dermatomyositis, mixed connectivetissue disease are characterized by the appearance of avariety of autoantibodies directed against componentsof the cell nucleus.Although significance and pathological relevance ofsome auto-antibodies are not completely revealed yet,the detection of auto-antibodies is widely establishedand plays an important role in the diagnosis of systemicautoimmune diseases.
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