The β1-adrenergic receptor is a G protein-coupled receptor. β receptors have the subtypes β1, β2 and β3. Agonist binding causes a rise in the intracellular concentration of the second messenger cAMP. The occurrence of auto- antibodies against the β1-adrenergic receptor is associated with the existence of dilated cardiomyopathy (DCM). Human anti-β1-adrenergic receptor antibody EIA is designed for the determination of antibodies (IgG) against the β1-adrenergic receptor in serum and plasma.
Optimal working dilution should be determined by the investigator.
Volume d'échantillon
100 μL
Durée du test
3.5 h
Protocole
Human anti-β1-adrenergic receptor antibody EIA is an antibody screening test. β1-adrenergic receptors have been pre-coated onto a microtiter plate. During the first incubation the anti-β1 adrenergic receptor antibodies of the samples are immobilized on the plate. The autoantibodies are detected with a HRP labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the color correlates with the concentration and/ or avidity of anti-β1-adrenergic receptor antibodies.