IL1A Kit ELISA
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- Antigène Voir toutes IL1A Kits ELISA
- IL1A (Interleukin 1 alpha (IL1A))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Application
- ELISA
- Fonction
- Mouse Interleukin 1 Alpha (IL-1α) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of mouse Interleukin 1 Alpha (IL-1α) concentrations in cell culture supernates, serum, and plasma.
- Type d'échantillon
- Serum, Plasma, Cell Culture Cells
- Analytical Method
- Quantitative
- Sensibilité
- 1 pg/ml
- Attributs du produit
- Mouse Interleukin 1 Alpha (IL-1α) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Volume d'échantillon
- 100 μL
- Durée du test
- 3.5 h
- Plaque
- Pre-coated
- Protocole
- Mouse Interleukin 1 Alpha (IL-1α) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-1α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1α present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-1α is added to the wells and binds to the combination of capture antibody-IL-1α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-1α present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-1α standard dilutions and IL-1α sample concentration determined.
- Restrictions
- For Research Use only
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- Stock
- 4 °C
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- Antigène Voir toutes IL1A Kits ELISA
- IL1A (Interleukin 1 alpha (IL1A))
- Autre désignation
- IL-1a (IL1A Produits)
- Synonymes
- IL-1A Kit ELISA, IL1 Kit ELISA, IL1-ALPHA Kit ELISA, IL1F1 Kit ELISA, IL-6 Kit ELISA, Il-1a Kit ELISA, IL-1 alpha Kit ELISA, IL-1alpha Kit ELISA, L1A Kit ELISA, interleukin 1 alpha Kit ELISA, interleukin 6 Kit ELISA, IL1A Kit ELISA, IL6 Kit ELISA, Il1a Kit ELISA
- Pathways
- Signalisation NF-kappaB, Autophagy, Cancer Immune Checkpoints
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